An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the natur...

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Bibliographic Details
Main Authors: Zhang, Hao, Ba, Sai, Co Co, Sarajane, Lee, Jasmine Yiqin, Guo, Juanjuan, Ye, Ruijuan, Liang, Zhaoxun, Huang, Dejian, Li, Tianhu
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
Subjects:
Oligonucleotides
DNA ligases
Online Access:https://hdl.handle.net/10356/82904
http://hdl.handle.net/10220/40359
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Institution: Nanyang Technological University
Language: English
Description
Summary:We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence.

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