An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the natur...

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Main Authors: Zhang, Hao, Ba, Sai, Co Co, Sarajane, Lee, Jasmine Yiqin, Guo, Juanjuan, Ye, Ruijuan, Liang, Zhaoxun, Huang, Dejian, Li, Tianhu
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2016
Subjects:
Online Access:https://hdl.handle.net/10356/82904
http://hdl.handle.net/10220/40359
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-829042020-03-07T12:18:08Z An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures Zhang, Hao Ba, Sai Co Co, Sarajane Lee, Jasmine Yiqin Guo, Juanjuan Ye, Ruijuan Liang, Zhaoxun Huang, Dejian Li, Tianhu School of Biological Sciences School of Physical and Mathematical Sciences Oligonucleotides DNA ligases We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence. MOE (Min. of Education, S’pore) 2016-03-31T09:21:07Z 2019-12-06T15:07:53Z 2016-03-31T09:21:07Z 2019-12-06T15:07:53Z 2015 Journal Article Zhang, H., Ba, S., Co Co, S., Lee, J. Y., Guo, J., Ye, R., et al. (2015). An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures. Bulletin of the Chemical Society of Japan, 88(9), 1314-1316. 0009-2673 https://hdl.handle.net/10356/82904 http://hdl.handle.net/10220/40359 10.1246/bcsj.20150120 en Bulletin of the Chemical Society of Japan © 2015 The Chemical Society of Japan.
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic Oligonucleotides
DNA ligases
spellingShingle Oligonucleotides
DNA ligases
Zhang, Hao
Ba, Sai
Co Co, Sarajane
Lee, Jasmine Yiqin
Guo, Juanjuan
Ye, Ruijuan
Liang, Zhaoxun
Huang, Dejian
Li, Tianhu
An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
description We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence.
author2 School of Biological Sciences
author_facet School of Biological Sciences
Zhang, Hao
Ba, Sai
Co Co, Sarajane
Lee, Jasmine Yiqin
Guo, Juanjuan
Ye, Ruijuan
Liang, Zhaoxun
Huang, Dejian
Li, Tianhu
format Article
author Zhang, Hao
Ba, Sai
Co Co, Sarajane
Lee, Jasmine Yiqin
Guo, Juanjuan
Ye, Ruijuan
Liang, Zhaoxun
Huang, Dejian
Li, Tianhu
author_sort Zhang, Hao
title An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
title_short An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
title_full An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
title_fullStr An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
title_full_unstemmed An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
title_sort alternative method for evaluating stabilities of dna hairpin structures
publishDate 2016
url https://hdl.handle.net/10356/82904
http://hdl.handle.net/10220/40359
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