NME proteins regulate class switch recombination

NME proteins regulate class switch recombination

Class switch recombination (CSR) in B cells involves deletion‐recombination at switch (S) region DNA and is important for the diversification of antibody isotypes during an immune response. Here, we identify two NME [NM23/NDPK (nucleoside diphosphate kinase)] isoforms, NME1 and NME2, as novel player...

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Bibliographic Details
Main Authors: Zheng, Simin, Kusnadi, Anthony, Choi, Jee Eun, Vuong, Bao Q., Rhodes, Daniela, Chaudhuri, Jayanta
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2019
Subjects:
DNA Recombination
G‐quadruplex
Science::Biological sciences
Online Access:https://hdl.handle.net/10356/86130
http://hdl.handle.net/10220/49849
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Institution: Nanyang Technological University
Language: English
Description
Summary:Class switch recombination (CSR) in B cells involves deletion‐recombination at switch (S) region DNA and is important for the diversification of antibody isotypes during an immune response. Here, we identify two NME [NM23/NDPK (nucleoside diphosphate kinase)] isoforms, NME1 and NME2, as novel players in this process. Knockdown of NME2 leads to decreased CSR, while knockdown of the highly homologous NME1 results in increased CSR. Interestingly, these NME proteins also display differential occupancy at S regions during CSR despite their homology; NME1 binds to S regions prior to stimulation, while NME2 binds to S regions only after stimulation. To the best of our knowledge, this represents the first report of a role for these proteins in the regulation of CSR.

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