Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins
Optical silencing of activity provides a way to test the necessity of neurons in behaviour. Two light-gated anion channels, GtACR1 and GtACR2, have recently been shown to potently inhibit activity in cultured mammalian neurons and in Drosophila. Here, we test the usefulness of these channels in larv...
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sg-ntu-dr.10356-866612020-11-01T05:16:07Z Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins Claridge-Chang, Adam Jesuthasan, Suresh Mohamed, Gadisti Aisha Cheng, Ruey-Kuang Ho, Joses Krishnan, Seetha Mohammad, Farhan Lee Kong Chian School of Medicine (LKCMedicine) Optogenetics Zebrafish Optical silencing of activity provides a way to test the necessity of neurons in behaviour. Two light-gated anion channels, GtACR1 and GtACR2, have recently been shown to potently inhibit activity in cultured mammalian neurons and in Drosophila. Here, we test the usefulness of these channels in larval zebrafish, using spontaneous coiling behaviour as the assay. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2017-12-20T08:10:26Z 2019-12-06T16:26:50Z 2017-12-20T08:10:26Z 2019-12-06T16:26:50Z 2017 Journal Article Mohamed, G. A., Cheng, R.-K., Ho, J., Krishnan, S., Mohammad, F., Claridge-Chang, et al. (2017). Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins. BMC Biology, 15(1), 103-. 1741-7007 https://hdl.handle.net/10356/86661 http://hdl.handle.net/10220/44172 10.1186/s12915-017-0430-2 en BMC Biology © 2017 The Author(s) (BioMed Central). This paper was published in BMC Biology and is made available as an electronic reprint (preprint) with permission of The Author(s) (BioMed Central). The published version is available at: [http://dx.doi.org/10.1186/s12915-017-0430-2]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 12 p. application/pdf |
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Optogenetics Zebrafish Claridge-Chang, Adam Jesuthasan, Suresh Mohamed, Gadisti Aisha Cheng, Ruey-Kuang Ho, Joses Krishnan, Seetha Mohammad, Farhan Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| description |
Optical silencing of activity provides a way to test the necessity of neurons in behaviour. Two light-gated anion channels, GtACR1 and GtACR2, have recently been shown to potently inhibit activity in cultured mammalian neurons and in Drosophila. Here, we test the usefulness of these channels in larval zebrafish, using spontaneous coiling behaviour as the assay. |
| author2 |
Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet |
Lee Kong Chian School of Medicine (LKCMedicine) Claridge-Chang, Adam Jesuthasan, Suresh Mohamed, Gadisti Aisha Cheng, Ruey-Kuang Ho, Joses Krishnan, Seetha Mohammad, Farhan |
| format |
Article |
| author |
Claridge-Chang, Adam Jesuthasan, Suresh Mohamed, Gadisti Aisha Cheng, Ruey-Kuang Ho, Joses Krishnan, Seetha Mohammad, Farhan |
| author_sort |
Claridge-Chang, Adam |
| title |
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| title_short |
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| title_full |
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| title_fullStr |
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| title_full_unstemmed |
Optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| title_sort |
optical inhibition of larval zebrafish behaviour with anion channelrhodopsins |
| publishDate |
2017 |
| url |
https://hdl.handle.net/10356/86661 http://hdl.handle.net/10220/44172 |
| _version_ |
1683493404368961536 |