De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis

Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes rema...

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Main Authors: Ding, Chunming, Lim, Yen Ching, Chia, Sook Yoong, Walet, Arcinas Camille Esther, Xu, Shaohai, Lo, Alice Kinyui, Zhao, Yanling, Zhu, Dewen, Shan, Zhihui, Chen, Qingfeng, Leow, Melvin Khee-Shing, Xu, Dan, Sun, Lei
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/87029
http://hdl.handle.net/10220/45300
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-870292023-12-29T06:45:15Z De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis Ding, Chunming Lim, Yen Ching Chia, Sook Yoong Walet, Arcinas Camille Esther Xu, Shaohai Lo, Alice Kinyui Zhao, Yanling Zhu, Dewen Shan, Zhihui Chen, Qingfeng Leow, Melvin Khee-Shing Xu, Dan Sun, Lei School of Chemical and Biomedical Engineering Adipose Brown Adipogenesis Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2018-07-27T04:26:50Z 2019-12-06T16:33:29Z 2018-07-27T04:26:50Z 2019-12-06T16:33:29Z 2018 Journal Article Ding, C., Lim, Y. C., Chia, S. Y., Walet, A. C. E., Xu, S., Lo, A. K., et al. (2018). De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nature Communications, 9(1), 1329-. https://hdl.handle.net/10356/87029 http://hdl.handle.net/10220/45300 10.1038/s41467-018-03754-3 en Nature Communications © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 14 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Adipose
Brown Adipogenesis
spellingShingle Adipose
Brown Adipogenesis
Ding, Chunming
Lim, Yen Ching
Chia, Sook Yoong
Walet, Arcinas Camille Esther
Xu, Shaohai
Lo, Alice Kinyui
Zhao, Yanling
Zhu, Dewen
Shan, Zhihui
Chen, Qingfeng
Leow, Melvin Khee-Shing
Xu, Dan
Sun, Lei
De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
description Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Ding, Chunming
Lim, Yen Ching
Chia, Sook Yoong
Walet, Arcinas Camille Esther
Xu, Shaohai
Lo, Alice Kinyui
Zhao, Yanling
Zhu, Dewen
Shan, Zhihui
Chen, Qingfeng
Leow, Melvin Khee-Shing
Xu, Dan
Sun, Lei
format Article
author Ding, Chunming
Lim, Yen Ching
Chia, Sook Yoong
Walet, Arcinas Camille Esther
Xu, Shaohai
Lo, Alice Kinyui
Zhao, Yanling
Zhu, Dewen
Shan, Zhihui
Chen, Qingfeng
Leow, Melvin Khee-Shing
Xu, Dan
Sun, Lei
author_sort Ding, Chunming
title De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
title_short De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
title_full De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
title_fullStr De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
title_full_unstemmed De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
title_sort de novo reconstruction of human adipose transcriptome reveals conserved lncrnas as regulators of brown adipogenesis
publishDate 2018
url https://hdl.handle.net/10356/87029
http://hdl.handle.net/10220/45300
_version_ 1787136433004216320