Allosteric pyruvate kinase-based “logic gate” synergistically senses energy and sugar levels in Mycobacterium tuberculosis

• Main Authors: Zhong, Wenhe, Cui, Liang, Goh, Boon Chong, Cai, Qixu, Ho, Peiying, Chionh, Yok Hian, Yuan, Meng, Sahili, Abbas El, Fothergill-Gilmore, Linda A., Walkinshaw, Malcolm D., Lescar, Julien, Dedon, Peter Christian
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2018
• Subjects: Mycobacterium Tuberculosis; Mycobacterium Bovis BCG
• Online Access: https://hdl.handle.net/10356/87429; http://hdl.handle.net/10220/44406
• Institution: Nanyang Technological University

Pyruvate kinase (PYK) is an essential glycolytic enzyme that controls glycolytic flux and is critical for ATP production in all organisms, with tight regulation by multiple metabolites. Yet the allosteric mechanisms governing PYK activity in bacterial pathogens are poorly understood. Here we report...