Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells

• Main Authors: Wang, Yuanming, Liu, Ivy Kaiwen, Sutrisnoh, Norfala-Aliah, Srinivasan, Harini, Zhang, Junyi, Li, Jia, Zhang, Fan, Lalith, Charles Richard John, Xing, Heyun, Shanmugam, Raghuvaran, Foo, Jia Nee, Yeo, Hwee Ting, Ooi, Kean Hean, Bleckwehl, Tore, Par, Rachel Yi Yun, Lee, Shi Mun, Ismail, Nur Nadiah, Sanwari, Nur Aidah, Lee, Vanessa Si Ting, Lew, Jan, Tan, Meng How
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2018
• Subjects: CRISPR; Genome Editing
• Online Access: https://hdl.handle.net/10356/87501; http://hdl.handle.net/10220/45417
• Institution: Nanyang Technological University

Background: While CRISPR-Cas systems hold tremendous potential for engineering the human genome, it is unclear how well each system performs against one another in both non-homologous end joining (NHEJ)-mediated and homology-directed repair (HDR)-mediated genome editing. Results: We systematically c...