A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat mo...

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Main Authors: Hou, Aihua, Bose, Tanima, Chandy, Kanianthara George, Tong, Louis
Other Authors: Lee Kong Chian School of Medicine (LKCMedicine)
Format: Article
Language:English
Published: 2018
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Online Access:https://hdl.handle.net/10356/88465
http://hdl.handle.net/10220/44618
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-884652020-11-01T05:30:39Z A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue Hou, Aihua Bose, Tanima Chandy, Kanianthara George Tong, Louis Lee Kong Chian School of Medicine (LKCMedicine) Immunology Dry Eye Disease Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat model. Lewis rats were immunized with an emulsion containing lacrimal gland extract, ovalbumin, and complete Freund's adjuvant. A second immunization with the same antigens in incomplete Freund's adjuvant was administered two weeks later. These immunizations were administered subcutaneously at the base of the tail. To boost the immune response at the ocular surface and lacrimal glands, lacrimal gland extract and ovalbumin were injected into the forniceal subconjunctiva and lacrimal glands 6 weeks after the first immunization. The rats developed dry eye features, including reduced tear production, decreased tear stability, and increased corneal damage. Immune profiling by flow cytometry showed a preponderance of CD3+ effector memory T cells in the eyeball. Published version 2018-03-27T03:58:46Z 2019-12-06T17:03:53Z 2018-03-27T03:58:46Z 2019-12-06T17:03:53Z 2017 Journal Article Hou, A., Bose, T., Chandy, K. G., & Tong, L. (2017). A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. Journal of Visualized Experiments, (124), e55592-. https://hdl.handle.net/10356/88465 http://hdl.handle.net/10220/44618 10.3791/55592 en Journal of Visualized Experiments © 2017 The Author(s) (Journal of Visualized Experiments). This paper was published under Creative Commons Attribution-Non Commercial-No Derivs 3.0 Unported Agreement in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of The Author(s) (Journal of Visualized Experiments). The published version is available at: [http://dx.doi.org/10.3791/55592]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 9 p. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic Immunology
Dry Eye Disease
spellingShingle Immunology
Dry Eye Disease
Hou, Aihua
Bose, Tanima
Chandy, Kanianthara George
Tong, Louis
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
description Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat model. Lewis rats were immunized with an emulsion containing lacrimal gland extract, ovalbumin, and complete Freund's adjuvant. A second immunization with the same antigens in incomplete Freund's adjuvant was administered two weeks later. These immunizations were administered subcutaneously at the base of the tail. To boost the immune response at the ocular surface and lacrimal glands, lacrimal gland extract and ovalbumin were injected into the forniceal subconjunctiva and lacrimal glands 6 weeks after the first immunization. The rats developed dry eye features, including reduced tear production, decreased tear stability, and increased corneal damage. Immune profiling by flow cytometry showed a preponderance of CD3+ effector memory T cells in the eyeball.
author2 Lee Kong Chian School of Medicine (LKCMedicine)
author_facet Lee Kong Chian School of Medicine (LKCMedicine)
Hou, Aihua
Bose, Tanima
Chandy, Kanianthara George
Tong, Louis
format Article
author Hou, Aihua
Bose, Tanima
Chandy, Kanianthara George
Tong, Louis
author_sort Hou, Aihua
title A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
title_short A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
title_full A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
title_fullStr A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
title_full_unstemmed A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
title_sort chronic autoimmune dry eye rat model with increase in effector memory t cells in eyeball tissue
publishDate 2018
url https://hdl.handle.net/10356/88465
http://hdl.handle.net/10220/44618
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