Electrical detection of hybridization and threading intercalation of deoxyribonucleic acid using carbon nanotube network field-effect transistors

• Main Authors: Rogers, John A., Gui, Ee Ling, Li, Lain-Jong, Lee, Pooi See, Lohani, Anup, Mhaisalkar, Subodh Gautam, Cao, Qing, Kang, Seong Jun, Tansil, Natalia Chendrawati, Gao, Zhiqiang
• Other Authors: School of Materials Science & Engineering
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Materials::Nanostructured materials
• Online Access: https://hdl.handle.net/10356/94252; http://hdl.handle.net/10220/8095
• Institution: Nanyang Technological University

The authors study deoxyribonucleic acid (DNA) sensing characteristics of carbon nanotube network field-effect transistors (CNNFETs) by monitoring their electrical responses upon immobilization with a DNA probe, hybridization with DNA analytes, and intercalation with a N,N'-bis(3-propylimidazole)-1,4,5,8-naphthalene diimide modified with Os(2,2'-bipyridine)2Cl+ pendants. The CNNFETs immobilized by single-stranded DNA molecules demonstrate the selective sensing of its complementary and single-base mismatched DNA (difference of ~16% in reduction of normalized drain current Id). Subsequent intercalation demonstrates a further sensitivity enhancement (difference of ~13% in Id reduction due to specific binding between hybridized DNA and intercalators, corroborated by the x-ray photoelectron spectroscopy study.