Nested PCR in magnetically actuated circular closed-loop PCR microchip system

• Main Authors: Lok, Khoi Seng, Lee, Peter Peng Foo, Kwok, Yien Chian, Nguyen, Nam-Trung
• Other Authors: School of Mechanical and Aerospace Engineering
• Format: Article
• Language: English
• Published: 2012
• Subjects: DRNTU::Engineering::Mechanical engineering
• Online Access: https://hdl.handle.net/10356/94315; http://hdl.handle.net/10220/7905
• Institution: Nanyang Technological University

We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of...