Nested PCR in magnetically actuated circular closed-loop PCR microchip system

• المؤلفون الرئيسيون: Lok, Khoi Seng, Lee, Peter Peng Foo, Kwok, Yien Chian, Nguyen, Nam-Trung
• مؤلفون آخرون: School of Mechanical and Aerospace Engineering
• التنسيق: مقال
• اللغة: English
• منشور في: 2012
• الموضوعات: DRNTU::Engineering::Mechanical engineering
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/94315; http://hdl.handle.net/10220/7905

We demonstrate a new and sensitive amplification technique (referred to as Nested Polymerase Chain Reaction; nPCR). It based on a magnetically actuated circular closed-loop PCR microchip system. nPCR involves the use of two sets of primers in two successive PCR runs, and allows the amplification of...