Exploring the energy landscape of nucleic acid hairpins using laser temperature-jump and microfluidic mixing

• Main Authors: Kuznetsov, Serguei V., Lapidus, Lisa J., Narayanan, Ranjani, Zhu, Li, Velmurugu, Yogambigai, Roca, Jorjethe, Prehna, Gerd, Ansari, Anjum
• Other Authors: School of Physical and Mathematical Sciences
• Format: Article
• Language: English
• Published: 2013
• Online Access: https://hdl.handle.net/10356/95816; http://hdl.handle.net/10220/11261
• Institution: Nanyang Technological University

We have investigated the multidimensionality of the free energy landscape accessible to a nucleic acid hairpin by measuring the relaxation kinetics in response to two very different perturbations of the folding/unfolding equilibrium, either a laser temperature-jump or ion-jump (from rapid mixing wit...