N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks
A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for...
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sg-ntu-dr.10356-974252020-03-07T12:18:19Z N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. School of Biological Sciences DRNTU::Science::Biological sciences A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin. 2013-06-25T02:18:11Z 2019-12-06T19:42:39Z 2013-06-25T02:18:11Z 2019-12-06T19:42:39Z 2011 2011 Journal Article Yang, R., Hou, W., Zhang, X., & Liu, C. F. (2012). N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks. Organic letters, 14(1), 374-377. 1523-7060 https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 10.1021/ol2031284 en Organic letters © 2011 American Chemical Society. |
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DRNTU::Science::Biological sciences Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| description |
A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin. |
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School of Biological Sciences |
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School of Biological Sciences Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. |
| format |
Article |
| author |
Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. |
| author_sort |
Liu, Chuan-Fa |
| title |
N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_short |
N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_full |
N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_fullStr |
N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_full_unstemmed |
N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_sort |
n-to-c sequential ligation using peptidyl n,n-bis(2-mercaptoethyl)amide building blocks |
| publishDate |
2013 |
| url |
https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 |
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1681046432871612416 |