N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks

• Main Authors: Liu, Chuan-Fa, Yang, Renliang., Hou, Wen., Zhang, Xiaohong.
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/97425; http://hdl.handle.net/10220/10577
• Institution: Nanyang Technological University

A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for...