N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks
A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for...
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Main Authors: | Liu, Chuan-Fa, Yang, Renliang., Hou, Wen., Zhang, Xiaohong. |
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Other Authors: | School of Biological Sciences |
Format: | Article |
Language: | English |
Published: |
2013
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Subjects: | |
Online Access: | https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 |
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Institution: | Nanyang Technological University |
Language: | English |
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