Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for monoclonal antibody expression level and quality in CHO cells

• Main Authors: Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng, Ho, Steven C. L.
• Other Authors: School of Chemical and Biomedical Engineering
• Format: Article
• Language: English
• Published: 2013
• Online Access: https://hdl.handle.net/10356/97890; http://hdl.handle.net/10220/13223
• Institution: Nanyang Technological University

Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on monoclonal antibody (mAb) expression level and qu...