Investigation of cell morphology by the TRUImagE digital holographic microscopy system

We report the development of the Three-dimensional Real-time Uninvasive Imaging and Evaluation (TRUImagE) system based on digital holographic microscopy to study the morphological changes in cells undergoing photodynamic therapyinduced cell death. The optical system, based on the Michelson interfero...

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Main Authors: Chee, Oi Choo, Qu, Weijuan, Chai, Kim Kheong, Asundi, Anand Krishna
Other Authors: School of Mechanical and Aerospace Engineering
Format: Conference or Workshop Item
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/98143
http://hdl.handle.net/10220/12314
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-981432023-03-04T17:07:26Z Investigation of cell morphology by the TRUImagE digital holographic microscopy system Chee, Oi Choo Qu, Weijuan Chai, Kim Kheong Asundi, Anand Krishna School of Mechanical and Aerospace Engineering Biophotonics: Photonic Solutions for Better Health Care (3rd : 2012 : Brussels, Belgium) DRNTU::Engineering::Mechanical engineering We report the development of the Three-dimensional Real-time Uninvasive Imaging and Evaluation (TRUImagE) system based on digital holographic microscopy to study the morphological changes in cells undergoing photodynamic therapyinduced cell death. The optical system, based on the Michelson interferometer and configured in transmission mode, and the sample holder incorporating a stage incubator have been developed for monitoring various tumorigenic cell samples without the use of markers. Off-axis digital holograms were recorded with a CCD sensor and numerically reconstructed to provide quantitative phase imaging and 3D morphology of the cells in real time. The system was used to continuously monitor and study, at different time points, the changes in cells after incubation with the photosensitizer followed by activation by the appropriate light dose. Results obtained from the TRUImagE system and biochemical assays will be given. 2013-07-25T08:45:50Z 2019-12-06T19:51:19Z 2013-07-25T08:45:50Z 2019-12-06T19:51:19Z 2012 2012 Conference Paper Chee, O. C., Qu, W., Chai, K. K., & Asundi, A. K. (2012). Investigation of cell morphology by the TRUImagE digital holographic microscopy system. Proceedings of SPIE - Biophotonics: Photonic Solutions for Better Health Care III, 84272G. https://hdl.handle.net/10356/98143 http://hdl.handle.net/10220/12314 10.1117/12.921613 en © 2012 SPIE. This paper was published in Proceedings of SPIE - Biophotonics: Photonic Solutions for Better Health Care III and is made available as an electronic reprint (preprint) with permission of SPIE. The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/12.921613]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf
institution Nanyang Technological University
building NTU Library
continent Asia
country Singapore
Singapore
content_provider NTU Library
collection DR-NTU
language English
topic DRNTU::Engineering::Mechanical engineering
spellingShingle DRNTU::Engineering::Mechanical engineering
Chee, Oi Choo
Qu, Weijuan
Chai, Kim Kheong
Asundi, Anand Krishna
Investigation of cell morphology by the TRUImagE digital holographic microscopy system
description We report the development of the Three-dimensional Real-time Uninvasive Imaging and Evaluation (TRUImagE) system based on digital holographic microscopy to study the morphological changes in cells undergoing photodynamic therapyinduced cell death. The optical system, based on the Michelson interferometer and configured in transmission mode, and the sample holder incorporating a stage incubator have been developed for monitoring various tumorigenic cell samples without the use of markers. Off-axis digital holograms were recorded with a CCD sensor and numerically reconstructed to provide quantitative phase imaging and 3D morphology of the cells in real time. The system was used to continuously monitor and study, at different time points, the changes in cells after incubation with the photosensitizer followed by activation by the appropriate light dose. Results obtained from the TRUImagE system and biochemical assays will be given.
author2 School of Mechanical and Aerospace Engineering
author_facet School of Mechanical and Aerospace Engineering
Chee, Oi Choo
Qu, Weijuan
Chai, Kim Kheong
Asundi, Anand Krishna
format Conference or Workshop Item
author Chee, Oi Choo
Qu, Weijuan
Chai, Kim Kheong
Asundi, Anand Krishna
author_sort Chee, Oi Choo
title Investigation of cell morphology by the TRUImagE digital holographic microscopy system
title_short Investigation of cell morphology by the TRUImagE digital holographic microscopy system
title_full Investigation of cell morphology by the TRUImagE digital holographic microscopy system
title_fullStr Investigation of cell morphology by the TRUImagE digital holographic microscopy system
title_full_unstemmed Investigation of cell morphology by the TRUImagE digital holographic microscopy system
title_sort investigation of cell morphology by the truimage digital holographic microscopy system
publishDate 2013
url https://hdl.handle.net/10356/98143
http://hdl.handle.net/10220/12314
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