Real-time determination of the activity of ATPase by use of a water-soluble polythiophene

• Main Authors: Umit, Hakan Yildiz, Chia, Wei Sheng, Diyar, Mailepessov, Chia, Diana Xueqi, Susana, Geifman Shochat, Bo, Liedberg
• Other Authors: School of Biological Sciences
• Format: Article
• Language: English
• Published: 2013
• Subjects: DRNTU::Science::Biological sciences
• Online Access: https://hdl.handle.net/10356/98531; http://hdl.handle.net/10220/10754
• Institution: Nanyang Technological University

This contribution introduces a fluorescence assay for real-time determination of the activity of p97/VCP, a 540-kDa homo-hexameric enzyme, belonging to the AAA-ATPase family. A fluorescent reporter “poly 1-(3-((4-methylthiophen-3-yl)oxy)propyl)quinuclidin-1-ium” (poly PTQ) is used to monitor the hyd...