Real-time determination of the activity of ATPase by use of a water-soluble polythiophene

This contribution introduces a fluorescence assay for real-time determination of the activity of p97/VCP, a 540-kDa homo-hexameric enzyme, belonging to the AAA-ATPase family. A fluorescent reporter “poly 1-(3-((4-methylthiophen-3-yl)oxy)propyl)quinuclidin-1-ium” (poly PTQ) is used to monitor the hyd...

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Bibliographic Details
Main Authors: Umit, Hakan Yildiz, Chia, Wei Sheng, Diyar, Mailepessov, Chia, Diana Xueqi, Susana, Geifman Shochat, Bo, Liedberg
Other Authors: School of Biological Sciences
Format: Article
Language:English
Published: 2013
Subjects:
Online Access:https://hdl.handle.net/10356/98531
http://hdl.handle.net/10220/10754
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Institution: Nanyang Technological University
Language: English

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