Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance

Microcapsules containing high-density biofilm-like Lactobacillus rhamnosus probiotics, in place of planktonic cells, are developed in order to enhance the cell viability upon exposures to stresses commonly encountered during food lifecycle (i.e., heating, freeze-drying, refrigerated storage, and aci...

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Main Authors: Cheow, Wean Sin, Hadinoto, Kunn
Other Authors: School of Chemical and Biomedical Engineering
Format: Article
Language:English
Published: 2013
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Online Access:https://hdl.handle.net/10356/99547
http://hdl.handle.net/10220/17626
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Institution: Nanyang Technological University
Language: English
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spelling sg-ntu-dr.10356-995472020-03-07T11:40:18Z Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance Cheow, Wean Sin Hadinoto, Kunn School of Chemical and Biomedical Engineering DRNTU::Science::Medicine::Biomedical engineering Microcapsules containing high-density biofilm-like Lactobacillus rhamnosus probiotics, in place of planktonic cells, are developed in order to enhance the cell viability upon exposures to stresses commonly encountered during food lifecycle (i.e., heating, freeze-drying, refrigerated storage, and acid). The high-density (HD) capsules are prepared by in situ cultivation of the planktonic cells in the confined space of polysaccharide-based capsules (i.e., chitosan-coated alginate and carrageenan capsules). Compared to their planktonic counterparts, the HD capsules exhibit higher freeze-drying resistance (40×) and higher thermotolerance upon prolonged wet heat exposures at 60 and 70 °C (12–8000×), but not at higher temperatures even for short exposures (i.e., 80 and 100 °C). The enhanced viability of the HD capsules, however, is not observed during the refrigerated storage and exposure to the simulated gastric juice. The alginate capsules are superior to carrageenan owed to their better cell release profile in the simulated intestinal juice and storage viability. 2013-11-14T04:11:45Z 2019-12-06T20:08:35Z 2013-11-14T04:11:45Z 2019-12-06T20:08:35Z 2013 2013 Journal Article Cheow, W. S., & Hadinoto, K. (2013). Biofilm-Like Lactobacillus rhamnosus Probiotics Encapsulated in Alginate and Carrageenan Microcapsules Exhibiting Enhanced Thermotolerance and Freeze-Drying Resistance. Biomacromolecules, 14(9), 3214-3222. https://hdl.handle.net/10356/99547 http://hdl.handle.net/10220/17626 10.1021/bm400853d en Biomacromolecules
institution Nanyang Technological University
building NTU Library
country Singapore
collection DR-NTU
language English
topic DRNTU::Science::Medicine::Biomedical engineering
spellingShingle DRNTU::Science::Medicine::Biomedical engineering
Cheow, Wean Sin
Hadinoto, Kunn
Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
description Microcapsules containing high-density biofilm-like Lactobacillus rhamnosus probiotics, in place of planktonic cells, are developed in order to enhance the cell viability upon exposures to stresses commonly encountered during food lifecycle (i.e., heating, freeze-drying, refrigerated storage, and acid). The high-density (HD) capsules are prepared by in situ cultivation of the planktonic cells in the confined space of polysaccharide-based capsules (i.e., chitosan-coated alginate and carrageenan capsules). Compared to their planktonic counterparts, the HD capsules exhibit higher freeze-drying resistance (40×) and higher thermotolerance upon prolonged wet heat exposures at 60 and 70 °C (12–8000×), but not at higher temperatures even for short exposures (i.e., 80 and 100 °C). The enhanced viability of the HD capsules, however, is not observed during the refrigerated storage and exposure to the simulated gastric juice. The alginate capsules are superior to carrageenan owed to their better cell release profile in the simulated intestinal juice and storage viability.
author2 School of Chemical and Biomedical Engineering
author_facet School of Chemical and Biomedical Engineering
Cheow, Wean Sin
Hadinoto, Kunn
format Article
author Cheow, Wean Sin
Hadinoto, Kunn
author_sort Cheow, Wean Sin
title Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
title_short Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
title_full Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
title_fullStr Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
title_full_unstemmed Biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
title_sort biofilm-like lactobacillus rhamnosus probiotics encapsulated in alginate and carrageenan microcapsules exhibiting enhanced thermotolerance and freeze-drying resistance
publishDate 2013
url https://hdl.handle.net/10356/99547
http://hdl.handle.net/10220/17626
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