Locked nucleic acid probe-based real-time PCR assay for the rapid detection of rifampin-resistant Mycobacterium tuberculosis

• Main Authors: Zhao Y., Li G., Sun C., Li C., Wang X., Liu H., Zhang P., Zhao X., Jiang Y., Yang R., Wan K., Zhou L.
• Other Authors: MEDICINE
• Format: Article
• Published: 2019
• Subjects: rifampicin; bacterial protein; DNA directed RNA polymerase; DNA probe; locked nucleic acid; oligonucleotide; rpoB protein, Mycobacterium tuberculosis; Article; bacterial gene; bacterial strain; bacterium detection; bacterium isolate; codon; controlled study; diagnostic value; DNA sequence; drug resistance; drug resistant tuberculosis; limit of detection; locked nucleic acid probe based real time polymerase chain reaction; molecular probe; multiplex polymerase chain reaction; mutational analysis; Mycobacterium tuberculosis; nonhuman; quantitative analysis; real time polymerase chain reaction; rpoB gene; sensitivity and specificity; sequence analysis; validation process; antibiotic resistance; drug effects; genetics; human; microbiology; mutation; procedures; reproducibility; Tuberculosis, Multidrug-Resistant; Bacterial Proteins; DNA Probes; DNA-Directed RNA Polymerases; Drug Resistance, Bacterial; Humans; Multiplex Polymerase Chain Reaction; Mutation; Oligonucleotides; Real-Time Polymerase Chain Reaction; Reproducibility of Results; Rifampin; Sensitivity and Specificity
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161603
• Institution: National University of Singapore

10.1371/journal.pone.0143444