Locked nucleic acid probe-based real-time PCR assay for the rapid detection of rifampin-resistant Mycobacterium tuberculosis

10.1371/journal.pone.0143444

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Bibliographic Details
Main Authors:	Zhao Y., Li G., Sun C., Li C., Wang X., Liu H., Zhang P., Zhao X., Jiang Y., Yang R., Wan K., Zhou L.
Other Authors:	MEDICINE
Format:	Article
Published:	2019
Subjects:	rifampicin bacterial protein DNA directed RNA polymerase DNA probe locked nucleic acid oligonucleotide rpoB protein, Mycobacterium tuberculosis Article bacterial gene bacterial strain bacterium detection bacterium isolate codon controlled study diagnostic value DNA sequence drug resistance drug resistant tuberculosis limit of detection locked nucleic acid probe based real time polymerase chain reaction molecular probe multiplex polymerase chain reaction mutational analysis Mycobacterium tuberculosis nonhuman quantitative analysis real time polymerase chain reaction rpoB gene sensitivity and specificity sequence analysis validation process antibiotic resistance drug effects genetics human microbiology mutation procedures reproducibility Tuberculosis, Multidrug-Resistant Bacterial Proteins DNA Probes DNA-Directed RNA Polymerases Drug Resistance, Bacterial Humans Multiplex Polymerase Chain Reaction Mutation Oligonucleotides Real-Time Polymerase Chain Reaction Reproducibility of Results Rifampin Sensitivity and Specificity
Online Access:	https://scholarbank.nus.edu.sg/handle/10635/161603
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Institution:	National University of Singapore

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