Toxoplasma gondii is dependent on glutamine and alters migratory profile of infected host bone marrow derived immune cells through SNAT2 and CXCR4 pathways

• Main Authors: Lee I.-P., Evans A.K., Yang C., Works M.G., Kumar V., De Miguel Z., Manley N.C., Sapolsky R.M.
• Other Authors: DEPT OF PHARMACOLOGY
• Format: Article
• Published: 2019
• Subjects: amino acid transporter; B7 antigen; chemokine receptor CXCR4; glutamine; lipopolysaccharide; phosphatidylinositol 3 kinase; Rho kinase; sodium dependent neutral amino acid transporter 2; stromal cell derived factor 1alpha; unclassified drug; CXCL12 protein, rat; Cxcr4 protein, rat; luciferase; SNAT2 protein, rat; stromal cell derived factor 1; amino acid transport; animal cell; animal tissue; Article; bone marrow; cell damage; cell migration; cell motility; controlled study; dendritic cell; female; host cell; immunocompetent cell; in vitro study; intracellular signaling; nonhuman; parasite cultivation; parasite viability; phenotype; protein expression; protein protein interaction; rat; Toxoplasma gondii; toxoplasmosis; Western blotting; animal; bone marrow cell; cell differentiation; cell motion; drug effects; fibroblast; gene expression regulation; genetics; host pathogen interaction; human; immunology; metabolism; newborn; parasitology; pathology; primary cell culture; reporter gene; signal transduction; Sprague Dawley rat; Toxoplasma; transgenic organism; Amino Acid Transport Systems; Animals; Animals, Newborn; Bone Marrow Cells; Cell Differentiation; Cell Movement; Chemokine CXCL12; Dendritic Cells; Fibroblasts; Gene Expression Regulation; Genes, Reporter; Glutamine; Host-Pathogen Interactions; Humans; Lipopolysaccharides; Luciferases; Organisms, Genetically Modified; Primary Cell Culture; Rats; Rats, Sprague-Dawley; Receptors, CXCR4; Signal Transduction
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161770
• Institution: National University of Singapore

10.1371/journal.pone.0109803