Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

• Main Authors: Hoelker M., Mekchay S., Schneider H., Bracket B.G., Tesfaye D., Jennen D., Tholen E., Gilles M., Rings F., Griese J., Schellander K.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-33847607522&partnerID=40&md5=429a4657c6d0436981f98d907d84539f; http://cmuir.cmu.ac.th/handle/6653943832/305
• Institution: Chiang Mai University

In this study, we compared the transfection effectiveness of liposomes with the new transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT). Furthermore, we examined whether plasmid architecture affects overall efficiency by comparing two plasmids, one of them bearing an addition...