Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

In this study, we compared the transfection effectiveness of liposomes with the new transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT). Furthermore, we examined whether plasmid architecture affects overall efficiency by comparing two plasmids, one of them bearing an addition...

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Bibliographic Details
Main Authors: Michael Hoelker, Supamit Mekchay, Hendrik Schneider, Benjamin Gaylord Bracket, Dawit Tesfaye, Danyel Jennen, Ernst Tholen, Markus Gilles, Franka Rings, Josef Griese, Karl Schellander
Format: Journal
Published: 2018
Subjects:
Agricultural and Biological Sciences
Veterinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847607522&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60826
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Institution: Chiang Mai University

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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847607522&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60826

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