Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

• Main Authors: Michael Hoelker, Supamit Mekchay, Hendrik Schneider, Benjamin Gaylord Bracket, Dawit Tesfaye, Danyel Jennen, Ernst Tholen, Markus Gilles, Franka Rings, Josef Griese, Karl Schellander
• 格式: 雜誌
• 出版: 2018
• 主題: Agricultural and Biological Sciences; Veterinary
• 在線閱讀: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847607522&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60826

In this study, we compared the transfection effectiveness of liposomes with the new transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT). Furthermore, we examined whether plasmid architecture affects overall efficiency by comparing two plasmids, one of them bearing an addition...