Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture
In this study, we compared the transfection effectiveness of liposomes with the new transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT). Furthermore, we examined whether plasmid architecture affects overall efficiency by comparing two plasmids, one of them bearing an addition...
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Main Authors: | Michael Hoelker, Supamit Mekchay, Hendrik Schneider, Benjamin Gaylord Bracket, Dawit Tesfaye, Danyel Jennen, Ernst Tholen, Markus Gilles, Franka Rings, Josef Griese, Karl Schellander |
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Format: | Journal |
Published: |
2018
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Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847607522&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60826 |
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Institution: | Chiang Mai University |
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