Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: Effect of transfection reagent and DNA architecture

In this study, we compared the transfection effectiveness of liposomes with the new transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT). Furthermore, we examined whether plasmid architecture affects overall efficiency by comparing two plasmids, one of them bearing an addition...

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Bibliographic Details
Main Authors: Hoelker M., Mekchay S., Schneider H., Bracket B.G., Tesfaye D., Jennen D., Tholen E., Gilles M., Rings F., Griese J., Schellander K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-33847607522&partnerID=40&md5=429a4657c6d0436981f98d907d84539f
http://cmuir.cmu.ac.th/handle/6653943832/305
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Institution: Chiang Mai University
Language: English

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