Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment

• Main Authors: Somsak V., Srichairatanakool S., Kamchonwongpaisan S., Yuthavong Y., Uthaipibull C.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-79953903955&partnerID=40&md5=9a4d34e211d4149fbc6265ce1365835c; http://cmuir.cmu.ac.th/handle/6653943832/3753
• Institution: Chiang Mai University

The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizonts. Here, protocols are described for small-scale in vitro cu...