Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice
© 2017, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved. Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisi...
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th-cmuir.6653943832-462762018-04-25T07:31:17Z Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice Noppawat Pengkumsri Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Sartjin Peerajan Periyanaina Kesika Khontaros Chaiyasut Chaiyavat Chaiyasut Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences © 2017, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved. Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisiae strain with high BG, extraction of BG using different chemical extraction methods, composition analysis of BG, and evaluation of the immunomodulatory effect of high-quality BG using mice model. Ten yeast strains were screened for high BG content using total glucan extraction kit and were subjected to FT-IR analysis. The kit based extraction revealed that HII31 showed a high content of total glucan and BG. HII31 cells were subjected to four different acid/base extractions, which indicated that combination of a strong base (NaOH) and weak acid (CH 3 COOOOH) extraction recovered high BG and a high ratio of polysaccharide, protein, and lipid. Further, the immunomodulatory effect of the selected BG was evaluated using mice, which suggested that low dose of HII31-BG induces the expression of selected pro-inflammatory (IL-17, IFN-γ) and anti-inflammatory cytokine (IL-10) significantly, whereas relatively high dose was required to alter the IL-6 and TGF-β expression. Overall, the present study revealed that BG extracted from HII31 cells alters the expression of studied cyto kines, which can be used as a potent immunomodulator in pharmaceutical products. 2018-04-25T06:52:11Z 2018-04-25T06:52:11Z 2017-01-01 Journal 1678457X 2-s2.0-85013836237 10.1590/1678-457X.10716 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85013836237&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46276 |
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Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences Noppawat Pengkumsri Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Sartjin Peerajan Periyanaina Kesika Khontaros Chaiyasut Chaiyavat Chaiyasut Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
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© 2017, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved. Beta-glucan (BG) is a conserved cell wall components of bacteria, fungi, and yeast. BG is an immunomodulator and stimulates the host immune system. This study was performed to screen Saccharomyces cerevisiae strain with high BG, extraction of BG using different chemical extraction methods, composition analysis of BG, and evaluation of the immunomodulatory effect of high-quality BG using mice model. Ten yeast strains were screened for high BG content using total glucan extraction kit and were subjected to FT-IR analysis. The kit based extraction revealed that HII31 showed a high content of total glucan and BG. HII31 cells were subjected to four different acid/base extractions, which indicated that combination of a strong base (NaOH) and weak acid (CH 3 COOOOH) extraction recovered high BG and a high ratio of polysaccharide, protein, and lipid. Further, the immunomodulatory effect of the selected BG was evaluated using mice, which suggested that low dose of HII31-BG induces the expression of selected pro-inflammatory (IL-17, IFN-γ) and anti-inflammatory cytokine (IL-10) significantly, whereas relatively high dose was required to alter the IL-6 and TGF-β expression. Overall, the present study revealed that BG extracted from HII31 cells alters the expression of studied cyto kines, which can be used as a potent immunomodulator in pharmaceutical products. |
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Journal |
author |
Noppawat Pengkumsri Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Sartjin Peerajan Periyanaina Kesika Khontaros Chaiyasut Chaiyavat Chaiyasut |
author_facet |
Noppawat Pengkumsri Bhagavathi Sundaram Sivamaruthi Sasithorn Sirilun Sartjin Peerajan Periyanaina Kesika Khontaros Chaiyasut Chaiyavat Chaiyasut |
author_sort |
Noppawat Pengkumsri |
title |
Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
title_short |
Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
title_full |
Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
title_fullStr |
Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
title_full_unstemmed |
Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice |
title_sort |
extraction of β-glucan from saccharomyces cerevisiae: comparison of different extraction methods and in vivo assessment of immunomodulatory effect in mice |
publishDate |
2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85013836237&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46276 |
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1681422844241641472 |