Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand

© 2017, Chiang Mai University. All rights reserved. Mango seeds are normally discarded as wastes, after processing of the mango pulp into different product, and accumulated more every year. Therefore, this study aimed to investigate the antioxidant activity of the mango seed kernel extracts (cultiva...

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Main Authors: Nichana Nakpanich, Wantida Chaiyana, Pimporn Leelapornpisid
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46409
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spelling th-cmuir.6653943832-464092018-04-25T07:25:45Z Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand Nichana Nakpanich Wantida Chaiyana Pimporn Leelapornpisid Chemistry Materials Science Mathematics Agricultural and Biological Sciences © 2017, Chiang Mai University. All rights reserved. Mango seeds are normally discarded as wastes, after processing of the mango pulp into different product, and accumulated more every year. Therefore, this study aimed to investigate the antioxidant activity of the mango seed kernel extracts (cultivar Keawmorakot and Mahachanok, both ripe and raw). The phytochemical constituents of the extracts were screened and phenolic compounds were observed in all extracts. Total phenolic contents of each extract were then determined using Folin-ciocalteu’s reagent, and the radicals scavenging activity of each extract was performed using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging assay, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)-radical scavenging assay. Linoleic acid peroxidation inhibition assay was evaluated using ferric thiocyanate method. The ripe Mahachanok extract (MS) exhibited the highest antioxidant activity in DPPH, ABTS-radical scavenging assay and linoleic acid peroxidation inhibition assay, with IC50 value of 8.055 ± 0.274 μg/mL, 3.215 ± 0.091 μg/mL, and 0.233 ± 0.021 μg/mL, respectively. The chemical profile of the mango seed kernel extracts was investigated using HPLC and showed that the major constituent of each extract was gallic acid, which was likely to be the major active compound that possesses the antioxidant activity. Furthermore, the stability of each extract in various conditions was performed in four conditions, which demonstrated that the antioxidant activity and the amount of gallic acid in all extracts were not significantly changed after storing at room temperature with light, room temperature in the dark, 45°C, and 4°C for 3 months. 2018-04-25T06:54:30Z 2018-04-25T06:54:30Z 2017-04-01 Journal 01252526 2-s2.0-85018509352 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018509352&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46409
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
spellingShingle Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
Nichana Nakpanich
Wantida Chaiyana
Pimporn Leelapornpisid
Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
description © 2017, Chiang Mai University. All rights reserved. Mango seeds are normally discarded as wastes, after processing of the mango pulp into different product, and accumulated more every year. Therefore, this study aimed to investigate the antioxidant activity of the mango seed kernel extracts (cultivar Keawmorakot and Mahachanok, both ripe and raw). The phytochemical constituents of the extracts were screened and phenolic compounds were observed in all extracts. Total phenolic contents of each extract were then determined using Folin-ciocalteu’s reagent, and the radicals scavenging activity of each extract was performed using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging assay, and 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)-radical scavenging assay. Linoleic acid peroxidation inhibition assay was evaluated using ferric thiocyanate method. The ripe Mahachanok extract (MS) exhibited the highest antioxidant activity in DPPH, ABTS-radical scavenging assay and linoleic acid peroxidation inhibition assay, with IC50 value of 8.055 ± 0.274 μg/mL, 3.215 ± 0.091 μg/mL, and 0.233 ± 0.021 μg/mL, respectively. The chemical profile of the mango seed kernel extracts was investigated using HPLC and showed that the major constituent of each extract was gallic acid, which was likely to be the major active compound that possesses the antioxidant activity. Furthermore, the stability of each extract in various conditions was performed in four conditions, which demonstrated that the antioxidant activity and the amount of gallic acid in all extracts were not significantly changed after storing at room temperature with light, room temperature in the dark, 45°C, and 4°C for 3 months.
format Journal
author Nichana Nakpanich
Wantida Chaiyana
Pimporn Leelapornpisid
author_facet Nichana Nakpanich
Wantida Chaiyana
Pimporn Leelapornpisid
author_sort Nichana Nakpanich
title Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
title_short Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
title_full Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
title_fullStr Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
title_full_unstemmed Antioxidant activities and stability of seed Kernel extracts from mango (Mangifera indica linn.) cultivated in Northern Thailand
title_sort antioxidant activities and stability of seed kernel extracts from mango (mangifera indica linn.) cultivated in northern thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018509352&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46409
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