The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics

© 2017 John Wiley & Sons Ltd The aim of the present study was to study the effects of different prebiotics (galacto-, fructooligosaccharide and inulin) on immune response and oxidative stress of common carp (Cyprinus carpio) fingerlings at the molecular level. A total number of 240 fish (13.85...

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Main Authors: Seyed Hossein Hoseinifar, Alireza Ahmadi, Mohsen Khalili, Mojtaba Raeisi, Hien Van Doan, Christopher Marlowe Caipang
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/56425
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-564252018-09-05T03:26:14Z The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics Seyed Hossein Hoseinifar Alireza Ahmadi Mohsen Khalili Mojtaba Raeisi Hien Van Doan Christopher Marlowe Caipang Agricultural and Biological Sciences © 2017 John Wiley & Sons Ltd The aim of the present study was to study the effects of different prebiotics (galacto-, fructooligosaccharide and inulin) on immune response and oxidative stress of common carp (Cyprinus carpio) fingerlings at the molecular level. A total number of 240 fish (13.85 ± 0.85 g) were supplied and randomly stocked in twelve fiberglass tanks (20 specimens per tank). Fish were fed a basal formulated diet (Control) or basal diet supplemented with equal level (2%) of different prebiotics (four treatments repeated in triplicated) for 8 weeks. At the end of feeding trial, the expression of immune-related genes (interleukin 1 beta [IL-1β], IL-8, IL-10, lysozyme [LYZ], tumour necrosis factor alpha [TNF-α] and transforming growth factor beta [TGF-β]) were determined in head kidney and intestine tissues and the expression of antioxidant-related genes (glutathione S-transferase [GST-α], glutathione reductase [GR] and glutathione peroxidase genes [GPX]) were studied in intestine. The results revealed that dietary administration of prebiotics modulated the expression of immune-related genes and the degree of expression was affected by the type of prebiotics and the organ that was used for analyses. Also, evaluation of antioxidant genes expression showed that GSTα and GR expression levels increased as a result of feeding common carp with the prebiotics. According to these findings, it can be concluded that feeding on different prebiotics had altered effects on the expression of immune and antioxidant-related genes. 2018-09-05T03:26:14Z 2018-09-05T03:26:14Z 2017-11-01 Journal 13652109 1355557X 2-s2.0-85018242303 10.1111/are.13359 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018242303&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56425
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Seyed Hossein Hoseinifar
Alireza Ahmadi
Mohsen Khalili
Mojtaba Raeisi
Hien Van Doan
Christopher Marlowe Caipang
The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
description © 2017 John Wiley & Sons Ltd The aim of the present study was to study the effects of different prebiotics (galacto-, fructooligosaccharide and inulin) on immune response and oxidative stress of common carp (Cyprinus carpio) fingerlings at the molecular level. A total number of 240 fish (13.85 ± 0.85 g) were supplied and randomly stocked in twelve fiberglass tanks (20 specimens per tank). Fish were fed a basal formulated diet (Control) or basal diet supplemented with equal level (2%) of different prebiotics (four treatments repeated in triplicated) for 8 weeks. At the end of feeding trial, the expression of immune-related genes (interleukin 1 beta [IL-1β], IL-8, IL-10, lysozyme [LYZ], tumour necrosis factor alpha [TNF-α] and transforming growth factor beta [TGF-β]) were determined in head kidney and intestine tissues and the expression of antioxidant-related genes (glutathione S-transferase [GST-α], glutathione reductase [GR] and glutathione peroxidase genes [GPX]) were studied in intestine. The results revealed that dietary administration of prebiotics modulated the expression of immune-related genes and the degree of expression was affected by the type of prebiotics and the organ that was used for analyses. Also, evaluation of antioxidant genes expression showed that GSTα and GR expression levels increased as a result of feeding common carp with the prebiotics. According to these findings, it can be concluded that feeding on different prebiotics had altered effects on the expression of immune and antioxidant-related genes.
format Journal
author Seyed Hossein Hoseinifar
Alireza Ahmadi
Mohsen Khalili
Mojtaba Raeisi
Hien Van Doan
Christopher Marlowe Caipang
author_facet Seyed Hossein Hoseinifar
Alireza Ahmadi
Mohsen Khalili
Mojtaba Raeisi
Hien Van Doan
Christopher Marlowe Caipang
author_sort Seyed Hossein Hoseinifar
title The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
title_short The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
title_full The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
title_fullStr The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
title_full_unstemmed The study of antioxidant enzymes and immune-related genes expression in common carp (Cyprinus carpio) fingerlings fed different prebiotics
title_sort study of antioxidant enzymes and immune-related genes expression in common carp (cyprinus carpio) fingerlings fed different prebiotics
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018242303&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56425
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