Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats

© 2016, The Japanese Society for Bone and Mineral Research and Springer Japan. Fibroblast growth factor (FGF)-21 is a potent endocrine factor that improves insulin resistance and obesity-associated metabolic disorders. However, concomitant activation of peroxisome proliferator-activated receptor-γ b...

Full description

Saved in:
Bibliographic Details
Main Authors: Narattaphol Charoenphandhu, Panan Suntornsaratoon, Nateetip Krishnamra, Piangkwan Sa-nguanmoo, Pongpun Tanajak, Xiaojie Wang, Guang Liang, Xiaokun Li, Chao Jiang, Nipon Chattipakorn, Siriporn Chattipakorn
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962293972&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56794
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-56794
record_format dspace
spelling th-cmuir.6653943832-567942018-09-05T03:49:07Z Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats Narattaphol Charoenphandhu Panan Suntornsaratoon Nateetip Krishnamra Piangkwan Sa-nguanmoo Pongpun Tanajak Xiaojie Wang Guang Liang Xiaokun Li Chao Jiang Nipon Chattipakorn Siriporn Chattipakorn Biochemistry, Genetics and Molecular Biology Medicine © 2016, The Japanese Society for Bone and Mineral Research and Springer Japan. Fibroblast growth factor (FGF)-21 is a potent endocrine factor that improves insulin resistance and obesity-associated metabolic disorders. However, concomitant activation of peroxisome proliferator-activated receptor-γ by FGF-21 makes bone susceptible to osteopenia and fragility fracture. Since an increase in body weight often induced adaptive change in bone by making it resistant to fracture, it was unclear whether FGF-21 would still induce bone defects in overweight rats. Therefore, the present study aimed to investigate bone microstructure and its mechanical properties in high fat diet (HF)-fed rats treated with 0.1 mg/kg/day FGF-21. Eighteen male rats were divided into two groups to receive either a normal diet or HF for 12 weeks. HF rats were then divided into two subgroups to receive either vehicle or FGF-21 for 4 weeks. The results showed that HF led to obesity, dyslipidemia and insulin resistance, as indicated by hyperinsulinemia with euglycemia. In HF rats, there was an increase in tibial yield displacement (an indicator of ability to be deformed without losing toughness, as determined by 3-point bending) without changes in tibial trabecular volumetric bone mineral density (vBMD) or cortical bone parameters, e.g., cortical thickness and bone area. FGF-21 treatment strongly improved the metabolic parameters and increased insulin sensitivity in HF rats. However, FGF-21-treated HF rats showed lower yield displacement, trabecular vBMD, trabecular bone volume, trabecular thickness, and osteoblast surface compared with vehicle-treated HF rats. These findings suggest that, despite being a potent antagonist of insulin resistance and visceral fat accumulation, FGF-21 is associated with bone defects in HF rats. 2018-09-05T03:30:20Z 2018-09-05T03:30:20Z 2017-03-01 Journal 14355604 09148779 2-s2.0-84962293972 10.1007/s00774-016-0745-z https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962293972&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/56794
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Narattaphol Charoenphandhu
Panan Suntornsaratoon
Nateetip Krishnamra
Piangkwan Sa-nguanmoo
Pongpun Tanajak
Xiaojie Wang
Guang Liang
Xiaokun Li
Chao Jiang
Nipon Chattipakorn
Siriporn Chattipakorn
Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
description © 2016, The Japanese Society for Bone and Mineral Research and Springer Japan. Fibroblast growth factor (FGF)-21 is a potent endocrine factor that improves insulin resistance and obesity-associated metabolic disorders. However, concomitant activation of peroxisome proliferator-activated receptor-γ by FGF-21 makes bone susceptible to osteopenia and fragility fracture. Since an increase in body weight often induced adaptive change in bone by making it resistant to fracture, it was unclear whether FGF-21 would still induce bone defects in overweight rats. Therefore, the present study aimed to investigate bone microstructure and its mechanical properties in high fat diet (HF)-fed rats treated with 0.1 mg/kg/day FGF-21. Eighteen male rats were divided into two groups to receive either a normal diet or HF for 12 weeks. HF rats were then divided into two subgroups to receive either vehicle or FGF-21 for 4 weeks. The results showed that HF led to obesity, dyslipidemia and insulin resistance, as indicated by hyperinsulinemia with euglycemia. In HF rats, there was an increase in tibial yield displacement (an indicator of ability to be deformed without losing toughness, as determined by 3-point bending) without changes in tibial trabecular volumetric bone mineral density (vBMD) or cortical bone parameters, e.g., cortical thickness and bone area. FGF-21 treatment strongly improved the metabolic parameters and increased insulin sensitivity in HF rats. However, FGF-21-treated HF rats showed lower yield displacement, trabecular vBMD, trabecular bone volume, trabecular thickness, and osteoblast surface compared with vehicle-treated HF rats. These findings suggest that, despite being a potent antagonist of insulin resistance and visceral fat accumulation, FGF-21 is associated with bone defects in HF rats.
format Journal
author Narattaphol Charoenphandhu
Panan Suntornsaratoon
Nateetip Krishnamra
Piangkwan Sa-nguanmoo
Pongpun Tanajak
Xiaojie Wang
Guang Liang
Xiaokun Li
Chao Jiang
Nipon Chattipakorn
Siriporn Chattipakorn
author_facet Narattaphol Charoenphandhu
Panan Suntornsaratoon
Nateetip Krishnamra
Piangkwan Sa-nguanmoo
Pongpun Tanajak
Xiaojie Wang
Guang Liang
Xiaokun Li
Chao Jiang
Nipon Chattipakorn
Siriporn Chattipakorn
author_sort Narattaphol Charoenphandhu
title Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
title_short Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
title_full Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
title_fullStr Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
title_full_unstemmed Fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
title_sort fibroblast growth factor-21 restores insulin sensitivity but induces aberrant bone microstructure in obese insulin-resistant rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84962293972&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56794
_version_ 1681424758561832960