Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity

The novel low-molecular-weight chitosan polysulfate (MW 5120-26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The...

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Main Authors: Suwan J., Zhang Z., Li B., Vongchan P., Meepowpan P., Zhang F., Mousa S.A., Mousa S., Premanode B., Kongtawelert P., Linhardt R.J.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-66649109421&partnerID=40&md5=672381a5fcbe04a59556100de1650ff7
http://www.ncbi.nlm.nih.gov/pubmed/19476923
http://cmuir.cmu.ac.th/handle/6653943832/5853
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-58532014-08-30T03:23:33Z Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity Suwan J. Zhang Z. Li B. Vongchan P. Meepowpan P. Zhang F. Mousa S.A. Mousa S. Premanode B. Kongtawelert P. Linhardt R.J. The novel low-molecular-weight chitosan polysulfate (MW 5120-26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay, and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight. © 2009 Elsevier Ltd. 2014-08-30T03:23:32Z 2014-08-30T03:23:32Z 2009 Article 00086215 10.1016/j.carres.2009.04.016 19476923 CRBRA http://www.scopus.com/inward/record.url?eid=2-s2.0-66649109421&partnerID=40&md5=672381a5fcbe04a59556100de1650ff7 http://www.ncbi.nlm.nih.gov/pubmed/19476923 http://cmuir.cmu.ac.th/handle/6653943832/5853 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The novel low-molecular-weight chitosan polysulfate (MW 5120-26,200 Da) was prepared using the depolymerization of chitosan with papain (EC. 3.4.22.2). The sulfonation of depolymerized products was performed using chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions. The structures of the products were characterized by FTIR, 13C NMR, and 1H NMR (1D, 2D NMR) spectroscopy. The present study sheds light on the mechanism of anticoagulant activity of chitosan polysulfate. Anticoagulant activity was investigated by an activated partial thromboplastin assay, a thrombin time assay, a prothrombin time assay, and thrombelastography. Surface plasmon resonance also provided valuable data for understanding the relationship between the molecular binding of sulfated chitosan to two important blood clotting regulators, antithrombin III and heparin cofactor II. These results show that the principal mechanism by which this chitosan polysulfate exhibits anticoagulant activity is mediated through heparin cofactor II and is dependent on polysaccharide molecular weight. © 2009 Elsevier Ltd.
format Article
author Suwan J.
Zhang Z.
Li B.
Vongchan P.
Meepowpan P.
Zhang F.
Mousa S.A.
Mousa S.
Premanode B.
Kongtawelert P.
Linhardt R.J.
spellingShingle Suwan J.
Zhang Z.
Li B.
Vongchan P.
Meepowpan P.
Zhang F.
Mousa S.A.
Mousa S.
Premanode B.
Kongtawelert P.
Linhardt R.J.
Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
author_facet Suwan J.
Zhang Z.
Li B.
Vongchan P.
Meepowpan P.
Zhang F.
Mousa S.A.
Mousa S.
Premanode B.
Kongtawelert P.
Linhardt R.J.
author_sort Suwan J.
title Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
title_short Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
title_full Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
title_fullStr Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
title_full_unstemmed Sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
title_sort sulfonation of papain-treated chitosan and its mechanism for anticoagulant activity
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-66649109421&partnerID=40&md5=672381a5fcbe04a59556100de1650ff7
http://www.ncbi.nlm.nih.gov/pubmed/19476923
http://cmuir.cmu.ac.th/handle/6653943832/5853
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