Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study

Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-m...

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Main Authors: Pongsri Tippawangkosol, Thitimonrat Duangchanda, Ratawan Ubalee, Ronnatrai Ruengweerayut, Kenji Hirayama, Kesara Na-Bangchang
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59909
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-599092018-09-10T03:23:26Z Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study Pongsri Tippawangkosol Thitimonrat Duangchanda Ratawan Ubalee Ronnatrai Ruengweerayut Kenji Hirayama Kesara Na-Bangchang Medicine Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-malaria exposed healthy adult volunteers were detected using the ex vivo ELISPOT assay. Five peptides from TRAP and CTRP antigens significantly increased IFN-γ responses of 1/9 in malaria-exposed volunteers. There is no statistically significant difference in positive T-cell response induced by any peptides in malaria exposed volunteers when evaluated as a group. The frequency of expressed HLA-A24 in malaria-exposed and non-malaria-exposed healthy adults living in northwest and central Thailand was 90% (27/30) and 100% (12/12), respectively. Although no association between positive T-cell response and HLA-A24 was found, due to the low number of positive responders achieved, one positive responder in malaria- exposed group was presented as HLA-24. 2018-09-10T03:23:26Z 2018-09-10T03:23:26Z 2009-01-01 Journal 01251562 2-s2.0-59149092814 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59149092814&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59909
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Pongsri Tippawangkosol
Thitimonrat Duangchanda
Ratawan Ubalee
Ronnatrai Ruengweerayut
Kenji Hirayama
Kesara Na-Bangchang
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
description Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-malaria exposed healthy adult volunteers were detected using the ex vivo ELISPOT assay. Five peptides from TRAP and CTRP antigens significantly increased IFN-γ responses of 1/9 in malaria-exposed volunteers. There is no statistically significant difference in positive T-cell response induced by any peptides in malaria exposed volunteers when evaluated as a group. The frequency of expressed HLA-A24 in malaria-exposed and non-malaria-exposed healthy adults living in northwest and central Thailand was 90% (27/30) and 100% (12/12), respectively. Although no association between positive T-cell response and HLA-A24 was found, due to the low number of positive responders achieved, one positive responder in malaria- exposed group was presented as HLA-24.
format Journal
author Pongsri Tippawangkosol
Thitimonrat Duangchanda
Ratawan Ubalee
Ronnatrai Ruengweerayut
Kenji Hirayama
Kesara Na-Bangchang
author_facet Pongsri Tippawangkosol
Thitimonrat Duangchanda
Ratawan Ubalee
Ronnatrai Ruengweerayut
Kenji Hirayama
Kesara Na-Bangchang
author_sort Pongsri Tippawangkosol
title Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
title_short Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
title_full Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
title_fullStr Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
title_full_unstemmed Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
title_sort identification of hla-a24 restricted pre-erythrocytic stage specific t-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59149092814&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59909
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