Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study
Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-m...
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th-cmuir.6653943832-599092018-09-10T03:23:26Z Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study Pongsri Tippawangkosol Thitimonrat Duangchanda Ratawan Ubalee Ronnatrai Ruengweerayut Kenji Hirayama Kesara Na-Bangchang Medicine Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-malaria exposed healthy adult volunteers were detected using the ex vivo ELISPOT assay. Five peptides from TRAP and CTRP antigens significantly increased IFN-γ responses of 1/9 in malaria-exposed volunteers. There is no statistically significant difference in positive T-cell response induced by any peptides in malaria exposed volunteers when evaluated as a group. The frequency of expressed HLA-A24 in malaria-exposed and non-malaria-exposed healthy adults living in northwest and central Thailand was 90% (27/30) and 100% (12/12), respectively. Although no association between positive T-cell response and HLA-A24 was found, due to the low number of positive responders achieved, one positive responder in malaria- exposed group was presented as HLA-24. 2018-09-10T03:23:26Z 2018-09-10T03:23:26Z 2009-01-01 Journal 01251562 2-s2.0-59149092814 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59149092814&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59909 |
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Medicine Pongsri Tippawangkosol Thitimonrat Duangchanda Ratawan Ubalee Ronnatrai Ruengweerayut Kenji Hirayama Kesara Na-Bangchang Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
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Fourteen (9 amino acids) peptides of Plasmodium falciparum pre-erythrocytic stage antigens, namely, TRAP, CTRP, LSA-1, STARP and MSP-1, restricted to HLA-A24 and specific to T-cell response were identified. The antigen-specific IFN-γ responses of these synthetic peptides in malaria exposed and non-malaria exposed healthy adult volunteers were detected using the ex vivo ELISPOT assay. Five peptides from TRAP and CTRP antigens significantly increased IFN-γ responses of 1/9 in malaria-exposed volunteers. There is no statistically significant difference in positive T-cell response induced by any peptides in malaria exposed volunteers when evaluated as a group. The frequency of expressed HLA-A24 in malaria-exposed and non-malaria-exposed healthy adults living in northwest and central Thailand was 90% (27/30) and 100% (12/12), respectively. Although no association between positive T-cell response and HLA-A24 was found, due to the low number of positive responders achieved, one positive responder in malaria- exposed group was presented as HLA-24. |
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Pongsri Tippawangkosol Thitimonrat Duangchanda Ratawan Ubalee Ronnatrai Ruengweerayut Kenji Hirayama Kesara Na-Bangchang |
author_facet |
Pongsri Tippawangkosol Thitimonrat Duangchanda Ratawan Ubalee Ronnatrai Ruengweerayut Kenji Hirayama Kesara Na-Bangchang |
author_sort |
Pongsri Tippawangkosol |
title |
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
title_short |
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
title_full |
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
title_fullStr |
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
title_full_unstemmed |
Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
title_sort |
identification of hla-a24 restricted pre-erythrocytic stage specific t-cell epitopes using plasmodium falciparum synthetic peptides: a preliminary study |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59149092814&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59909 |
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