L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources

One hundred and twenty four isolates of acidotolerant acetic acid bacteria, isolated from various kinds of fruits and flowers collected in Chiang Mai, Thailand by an enrichmentculture approach were screened for their ability to produce rare ketose sugars. It was found that only 40 isolates (∼32%) co...

Full description

Saved in:
Bibliographic Details
Main Authors: Watcharee Hanmoungjai, Ekachai Chukeatirote, Yuzo Yamada, Verasak Sahachaisaree, Pipob Lumyong, Goro Takata, Ken Izumori, Saisamorn Lumyong
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650323952&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60174
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-60174
record_format dspace
spelling th-cmuir.6653943832-601742018-09-10T03:48:39Z L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources Watcharee Hanmoungjai Ekachai Chukeatirote Yuzo Yamada Verasak Sahachaisaree Pipob Lumyong Goro Takata Ken Izumori Saisamorn Lumyong Biochemistry, Genetics and Molecular Biology Chemistry Materials Science Mathematics Physics and Astronomy One hundred and twenty four isolates of acidotolerant acetic acid bacteria, isolated from various kinds of fruits and flowers collected in Chiang Mai, Thailand by an enrichmentculture approach were screened for their ability to produce rare ketose sugars. It was found that only 40 isolates (∼32%) could produce L-sorbose from sorbitol as detected by cysteinecarbazole reaction. This was then confirmed based on the thin layer chromatography technique. However, when analysed by HPLC, only twenty-two isolates were capable of producing L-sorbose. Of these, the strain CMU 48-1 produced highest accumulation of L-sorbose (0.629%), and was identified as Gluconacetobacter liquefaciens based on 16S rRNA gene sequence analysis. This is the first report of biotransformation of L-sorbose from sorbitol by Gluconacetobacter liquefaciens. 2018-09-10T03:39:00Z 2018-09-10T03:39:00Z 2008-05-01 Journal 01252526 2-s2.0-67650323952 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650323952&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60174
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Materials Science
Mathematics
Physics and Astronomy
Watcharee Hanmoungjai
Ekachai Chukeatirote
Yuzo Yamada
Verasak Sahachaisaree
Pipob Lumyong
Goro Takata
Ken Izumori
Saisamorn Lumyong
L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
description One hundred and twenty four isolates of acidotolerant acetic acid bacteria, isolated from various kinds of fruits and flowers collected in Chiang Mai, Thailand by an enrichmentculture approach were screened for their ability to produce rare ketose sugars. It was found that only 40 isolates (∼32%) could produce L-sorbose from sorbitol as detected by cysteinecarbazole reaction. This was then confirmed based on the thin layer chromatography technique. However, when analysed by HPLC, only twenty-two isolates were capable of producing L-sorbose. Of these, the strain CMU 48-1 produced highest accumulation of L-sorbose (0.629%), and was identified as Gluconacetobacter liquefaciens based on 16S rRNA gene sequence analysis. This is the first report of biotransformation of L-sorbose from sorbitol by Gluconacetobacter liquefaciens.
format Journal
author Watcharee Hanmoungjai
Ekachai Chukeatirote
Yuzo Yamada
Verasak Sahachaisaree
Pipob Lumyong
Goro Takata
Ken Izumori
Saisamorn Lumyong
author_facet Watcharee Hanmoungjai
Ekachai Chukeatirote
Yuzo Yamada
Verasak Sahachaisaree
Pipob Lumyong
Goro Takata
Ken Izumori
Saisamorn Lumyong
author_sort Watcharee Hanmoungjai
title L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
title_short L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
title_full L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
title_fullStr L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
title_full_unstemmed L-sorbose production by acidotolerant acetic acid bacteria isolated from Thailand sources
title_sort l-sorbose production by acidotolerant acetic acid bacteria isolated from thailand sources
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650323952&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60174
_version_ 1681425387126521856