Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism

Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism

Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan®and LightCycler™ for the pharmacogenetic evaluation of CYP2B6 516G...

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Main Authors: Soranun Chantarangsu, Tim Cressey, Surakameth Mahasirimongkol, Yardpiroon Tawon, Nicole Ngo-Giang-Huong, Gonzague Jourdain, Marc Lallemant, Wasun Chantratita
Format: Journal
Published: 2018
Subjects:
Biochemistry, Genetics and Molecular Biology
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547928361&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60884
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-608842018-09-10T04:00:48Z Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism Soranun Chantarangsu Tim Cressey Surakameth Mahasirimongkol Yardpiroon Tawon Nicole Ngo-Giang-Huong Gonzague Jourdain Marc Lallemant Wasun Chantratita Biochemistry, Genetics and Molecular Biology Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan®and LightCycler™ for the pharmacogenetic evaluation of CYP2B6 516G>T polymorphism. Both methods were found to be suitable for pharmacogenetic testing of CYP2B6 516G>T in the meaning of time consumption, accurate genotype calling, flexible reaction format, simple data analysis, and low cost per assay. The genotype success rate was similar, but the LightCycler procedure was less expensive in terms of cost per sample and shorter running time. © 2007 Elsevier Ltd. All rights reserved. 2018-09-10T04:00:48Z 2018-09-10T04:00:48Z 2007-10-01 Journal 08908508 2-s2.0-34547928361 10.1016/j.mcp.2007.06.008 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547928361&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60884
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
Soranun Chantarangsu
Tim Cressey
Surakameth Mahasirimongkol
Yardpiroon Tawon
Nicole Ngo-Giang-Huong
Gonzague Jourdain
Marc Lallemant
Wasun Chantratita
Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
description Understanding genetically encoded inherited differences in drug metabolism and drug targets offers the promise of minimizing adverse drug reactions and improving therapies. We have compared two real-time PCR-based methods, the TaqMan®and LightCycler™ for the pharmacogenetic evaluation of CYP2B6 516G>T polymorphism. Both methods were found to be suitable for pharmacogenetic testing of CYP2B6 516G>T in the meaning of time consumption, accurate genotype calling, flexible reaction format, simple data analysis, and low cost per assay. The genotype success rate was similar, but the LightCycler procedure was less expensive in terms of cost per sample and shorter running time. © 2007 Elsevier Ltd. All rights reserved.
format Journal
author Soranun Chantarangsu
Tim Cressey
Surakameth Mahasirimongkol
Yardpiroon Tawon
Nicole Ngo-Giang-Huong
Gonzague Jourdain
Marc Lallemant
Wasun Chantratita
author_facet Soranun Chantarangsu
Tim Cressey
Surakameth Mahasirimongkol
Yardpiroon Tawon
Nicole Ngo-Giang-Huong
Gonzague Jourdain
Marc Lallemant
Wasun Chantratita
author_sort Soranun Chantarangsu
title Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
title_short Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
title_full Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
title_fullStr Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
title_full_unstemmed Comparison of the TaqMan and LightCycler systems in evaluation of CYP2B6 516G>T polymorphism
title_sort comparison of the taqman and lightcycler systems in evaluation of cyp2b6 516g>t polymorphism
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34547928361&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60884
_version_ 1681425518306525184

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