Molecular Cloning and Sequencing of Raw Starch Degrading Gene from Laceyella sacchari LP175 and Its Functional Expression in Escherichia coli

• Main Authors: Thanasak Lomthong, Sunee Chotineeranat, Gianluca Cioci, Elisabeth Laville, Sophie Duquesne, Kiattawee Choowongkomon, Alain Marty, Vichien Kitpreechavanich
• Format: บทความวารสาร
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9308; http://cmuir.cmu.ac.th/jspui/handle/6653943832/64147
• Institution: Chiang Mai University

Raw starch degrading enzyme (RSDE) produced by Laceyella sacchari LP175 was purified 14.7 fold to a 40.5 % yield. The first 15 N-terminal amino acids were sequenced and showed 100% homology with α-amylase from Laceyella sp. DS3 and Thermoactinomyces vulgaris. The RSDE gene was functionally annotated...