Molecular Cloning and Sequencing of Raw Starch Degrading Gene from Laceyella sacchari LP175 and Its Functional Expression in Escherichia coli
Raw starch degrading enzyme (RSDE) produced by Laceyella sacchari LP175 was purified 14.7 fold to a 40.5 % yield. The first 15 N-terminal amino acids were sequenced and showed 100% homology with α-amylase from Laceyella sp. DS3 and Thermoactinomyces vulgaris. The RSDE gene was functionally annotated...
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| Main Authors: | , , , , , , , |
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| Format: | บทความวารสาร |
| Language: | English |
| Published: |
Science Faculty of Chiang Mai University
2019
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| Online Access: | http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=9308 http://cmuir.cmu.ac.th/jspui/handle/6653943832/64147 |
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| Institution: | Chiang Mai University |
| Language: | English |