Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia

© 2019 Elsevier B.V. The aim of the present study was to determine the effects of dietary arginine supplementation on resistance and responses to ammonia toxicity in common carp (Cyprinus carpio). In the first experiment, the fish were fed with four diets supplemented with 0% (control diet), 0.25% (...

Full description

Saved in:
Bibliographic Details
Main Authors: Seyyed Morteza Hoseini, Yury Anatolyevich Vatnikov, Evgeny Vladimirovich Kulikov, Alexander Konstantinovich Petrov, Seyed Hossein Hoseinifar, Hien Van Doan
Format: Journal
Published: 2019
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85067646311&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65230
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-65230
record_format dspace
spelling th-cmuir.6653943832-652302019-08-05T04:30:47Z Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia Seyyed Morteza Hoseini Yury Anatolyevich Vatnikov Evgeny Vladimirovich Kulikov Alexander Konstantinovich Petrov Seyed Hossein Hoseinifar Hien Van Doan Agricultural and Biological Sciences © 2019 Elsevier B.V. The aim of the present study was to determine the effects of dietary arginine supplementation on resistance and responses to ammonia toxicity in common carp (Cyprinus carpio). In the first experiment, the fish were fed with four diets supplemented with 0% (control diet), 0.25% (0.25Arg), 0.5% (0.5Arg) or 1% (1Arg) for 14 days and challenged with 0.7, 0.8, 0.9, 1.1, 1.3 mg/L water unionized ammonia for 3 h. The results showed that 0.7 mg/L ammonia caused no mortality in the treatments, but 1.3 mg/L ammonia killed all the fish. In the second experiment, the fish fed either the control or the 0.5Arg diet for 14 days and exposed to 0.7 mg/L ammonia for 3 h, and plasma ammonia and free amino acids were determined. The results showed that arginine administration significantly increased plasma arginine, urea, ornithine, glutamate, aspartate and histidine, whereas decreased plasma ammonia, citrulline, glutamine and other amino acids except for glycine, asparagine and serine. Ammonia exposure led to significant elevations in plasma ammonia and urea, and significant declines in plasma arginine, ornithine, citrulline and other amino acids. In conclusion, common carp is ureagenic under ammonia exposure and tries to detoxify ammonia by converting to urea, as well as formation of glutamine. To keep glutamate levels constant during ammonia toxicity, common carp may utilize alanine, aspartate, glycine and serine for α-ketoglutarate formation as a substrate for glutamate synthesis. Arginine administration provides high levels of ornithine, which is used to convert ammonia to urea after ammonia toxicity. The benefits of arginine in common carp resistance to ammonia toxicity seem to be related to its stimulatory effects on ureageneis; however, other health benefits of the amino acid (anti-stress, antioxidant and anti-anemic) should not be neglected. 2019-08-05T04:30:47Z 2019-08-05T04:30:47Z 2019-09-15 Journal 00448486 2-s2.0-85067646311 10.1016/j.aquaculture.2019.734209 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85067646311&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/65230
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Seyyed Morteza Hoseini
Yury Anatolyevich Vatnikov
Evgeny Vladimirovich Kulikov
Alexander Konstantinovich Petrov
Seyed Hossein Hoseinifar
Hien Van Doan
Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
description © 2019 Elsevier B.V. The aim of the present study was to determine the effects of dietary arginine supplementation on resistance and responses to ammonia toxicity in common carp (Cyprinus carpio). In the first experiment, the fish were fed with four diets supplemented with 0% (control diet), 0.25% (0.25Arg), 0.5% (0.5Arg) or 1% (1Arg) for 14 days and challenged with 0.7, 0.8, 0.9, 1.1, 1.3 mg/L water unionized ammonia for 3 h. The results showed that 0.7 mg/L ammonia caused no mortality in the treatments, but 1.3 mg/L ammonia killed all the fish. In the second experiment, the fish fed either the control or the 0.5Arg diet for 14 days and exposed to 0.7 mg/L ammonia for 3 h, and plasma ammonia and free amino acids were determined. The results showed that arginine administration significantly increased plasma arginine, urea, ornithine, glutamate, aspartate and histidine, whereas decreased plasma ammonia, citrulline, glutamine and other amino acids except for glycine, asparagine and serine. Ammonia exposure led to significant elevations in plasma ammonia and urea, and significant declines in plasma arginine, ornithine, citrulline and other amino acids. In conclusion, common carp is ureagenic under ammonia exposure and tries to detoxify ammonia by converting to urea, as well as formation of glutamine. To keep glutamate levels constant during ammonia toxicity, common carp may utilize alanine, aspartate, glycine and serine for α-ketoglutarate formation as a substrate for glutamate synthesis. Arginine administration provides high levels of ornithine, which is used to convert ammonia to urea after ammonia toxicity. The benefits of arginine in common carp resistance to ammonia toxicity seem to be related to its stimulatory effects on ureageneis; however, other health benefits of the amino acid (anti-stress, antioxidant and anti-anemic) should not be neglected.
format Journal
author Seyyed Morteza Hoseini
Yury Anatolyevich Vatnikov
Evgeny Vladimirovich Kulikov
Alexander Konstantinovich Petrov
Seyed Hossein Hoseinifar
Hien Van Doan
author_facet Seyyed Morteza Hoseini
Yury Anatolyevich Vatnikov
Evgeny Vladimirovich Kulikov
Alexander Konstantinovich Petrov
Seyed Hossein Hoseinifar
Hien Van Doan
author_sort Seyyed Morteza Hoseini
title Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
title_short Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
title_full Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
title_fullStr Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
title_full_unstemmed Effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (Cyprinus carpio) exposed to ambient ammonia
title_sort effects of dietary arginine supplementation on ureagenesis and amino acid metabolism in common carp (cyprinus carpio) exposed to ambient ammonia
publishDate 2019
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85067646311&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65230
_version_ 1681426230005465088