Enhanced Production of Phytase, A Feed Enzyme, from Pichia kudriavzevii using Mutagenesis and Improved Culture Conditions

• Main Authors: Wanatchaporn Boontham, Natsuda Srivanichpoom, Pumin Nutaratat, Savitree Limtong, Nantana Srisuk
• Language: English
• Published: Science Faculty of Chiang Mai University 2019
• Subjects: phytase; optimisation; mutagenesis; Pichia kudriavzevii; yeast
• Online Access: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=10134; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66022
• Institution: Chiang Mai University

Pichia kudriavzevii WB17-1, a phytase-producing yeast isolated from duck excrement, was found to produce both cell-bound and extracellular phytases. To enhance extracellular phytase, P. kudriavzevii WB17-1 was subjected to induced mutation. Ethylmethane sulfonate (EMS)-induced mutation resulted in 2...