Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate
Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previous...
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Chiang Mai University
2020
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th-cmuir.6653943832-686672020-05-20T04:41:50Z Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate Patwarin Tohmadlae Wanchai Worawattanamateekul irapa Hinsui Protease Tuna pepsin Collagen hydrolysate Fish skin Tilapia skin Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previously published or simultaneously submitted for publication. Manuscripts are peer reviewed using the double -blinded review system by at least 2 reviewers before acceptance. The CMU Journal of Natural Sciences is published four times a year, in January, April, July and October. Thailand is a leading exporter of canned tuna globally. Many by-products are created during processing, including head, bone, blood and stomach. The stomach can serve as a promising source of pepsin, while collagen hydrolysate can be obtained as a new value-added product with high market value. The objectives of this study were to characterize pepsin from tuna stomachs and evaluate its application for extraction of collagen hydrolysate from tilapia skin. Pepsin from the stomachs of albacore tuna, skipjack tuna, and yellowfin tuna was characterized. Pepsin from all tuna species was extracted with phosphate buffer (pH 7) at 4°C for 3 h then mixed with 2 M acetic acid at 1:1 (w/v) for 30 minutes. The characterization of crude enzyme was determined. The optimum pH of all tuna pepsin was 2, and stable at pH2-3. Optimum temperature of all tuna pepsin was 50 °C, and it was stable at 10-50 °C. This enzyme responded to EDTA, urea, copper sulfate and magnesium sulfate. Albacore tuna (3.52±1.09 unit/ml), skipjack tuna (3.42±1.008 unit/ml), yellowfin tuna (3.51±0.29 unit/ml) and porcine pepsin (3.96±0.00 unit/ml) were applied for collagen hydrolysate extraction at 50 °C for 0-3 h. Degree of hydrolysis (%DH) of yellowfin tuna pepsin was highest (75.99±0.02%) at 50 °C for 1 h. Collagen hydrolysate showed antioxidant properties (DPPH, ABTS and FRAP). Yellowfin tuna pepsin can be applied in food supplement production as well to commercial porcine pepsin. 2020-05-20T04:41:50Z 2020-05-20T04:41:50Z 2020 Chiang Mai University (CMU) Journal of Natural Sciences 19,2 (Apr-Jun 2020), p.206-221 2465-4337 http://cmuj.cmu.ac.th/uploads/journal_list_index/680696849.pdf http://cmuir.cmu.ac.th/jspui/handle/6653943832/68667 Eng Chiang Mai University |
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Thailand Thailand |
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CMU Intellectual Repository |
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English |
| topic |
Protease Tuna pepsin Collagen hydrolysate Fish skin Tilapia skin |
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Protease Tuna pepsin Collagen hydrolysate Fish skin Tilapia skin Patwarin Tohmadlae Wanchai Worawattanamateekul irapa Hinsui Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| description |
Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previously published or simultaneously submitted for publication. Manuscripts are peer reviewed using the double -blinded review system by at least 2 reviewers before acceptance. The CMU Journal of Natural Sciences is published four times a year, in January, April, July and October. |
| author |
Patwarin Tohmadlae Wanchai Worawattanamateekul irapa Hinsui |
| author_facet |
Patwarin Tohmadlae Wanchai Worawattanamateekul irapa Hinsui |
| author_sort |
Patwarin Tohmadlae |
| title |
Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| title_short |
Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| title_full |
Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| title_fullStr |
Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| title_full_unstemmed |
Characterization of Acidic Tuna Protease and Its Application for Extraction of Tilapia Collagen Hydrolysate |
| title_sort |
characterization of acidic tuna protease and its application for extraction of tilapia collagen hydrolysate |
| publisher |
Chiang Mai University |
| publishDate |
2020 |
| url |
http://cmuj.cmu.ac.th/uploads/journal_list_index/680696849.pdf http://cmuir.cmu.ac.th/jspui/handle/6653943832/68667 |
| _version_ |
1681752673585463296 |