Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science...
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Faculty of Science, Chiang Mai University
2020
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th-cmuir.6653943832-699122020-10-08T08:36:17Z Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates Lili Liu Ke Chen Mengjun Zhang Shengjuan Shi Weiwei Cheng Bacillus cereus MBL13 collagenolytic protease bovine bone collagen peptide chelating calcium chemical properties structural properties The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so. In order to utilize bovine bone byproducts in a calcium supplement with high solubility, bovine bone was hydrolyzed by a specific bone-degrading collagenolytic protease extracted from Bacillus cereus MBL13 (isolated from chopped animal bone wastes). A calcium-binding bovine bone peptide (BBP) was successfully purified from the bone hydrolysate. Its amino acid sequence was identified as Phe-Glu-Ser-Asn-Phe-Asn-Thr-Gln-Ala-Thr-Asn-Arg (MW: 1428 Da) through ESI-QTOF tandem mass analysis. The chemical and structural properties of the BBP chelating calcium (BBP-Ca) were investigated. The results proved that BBP-Ca was rich in peptide and calcium, and exhibited excellent thermal solubility and high solubility under either acidic or basic conditions, which were beneficial to be absorbed and transported in the gastrointestinal tracts of humans. Analysis of ultraviolet spectroscopy, fluorescence spectroscopy and Fourier transform infrared spectroscopy suggested that carboxyl groups, amino groups, and carbonyl groups of BBP were the primary chelating calcium sites. Raman spectroscopy showed that the secondary structure of BBP-Ca mainly contained β- folding and random coil. Meanwhile, X-ray diffraction spectra demonstrated that the irregular non-crystal structure of BBP turned into the crystal structure after chelated with calcium. Moreover, SEM indicated that there was a certain degree of adsorption besides the coordinate binding and ion binding between BBP and calcium. Therefore, BBP-Ca was a new and stable peptide-calcium chelate by the analysis of its chemical and structural properties. This research provides a theoretical basis for the development of new calcium nutraceutical additives. 2020-10-08T08:36:17Z 2020-10-08T08:36:17Z 2020 Chiang Mai Journal of Science 47,5 (Sep 2020), p.943-957 2465-3845 https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11193 http://cmuir.cmu.ac.th/jspui/handle/6653943832/69912 Eng Faculty of Science, Chiang Mai University |
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Bacillus cereus MBL13 collagenolytic protease bovine bone collagen peptide chelating calcium chemical properties structural properties |
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Bacillus cereus MBL13 collagenolytic protease bovine bone collagen peptide chelating calcium chemical properties structural properties Lili Liu Ke Chen Mengjun Zhang Shengjuan Shi Weiwei Cheng Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
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The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so. |
author |
Lili Liu Ke Chen Mengjun Zhang Shengjuan Shi Weiwei Cheng |
author_facet |
Lili Liu Ke Chen Mengjun Zhang Shengjuan Shi Weiwei Cheng |
author_sort |
Lili Liu |
title |
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
title_short |
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
title_full |
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
title_fullStr |
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
title_full_unstemmed |
Preparation and Characterization of a Novel Peptide Chelating Calcium from Bovine Bone Hydrolysates |
title_sort |
preparation and characterization of a novel peptide chelating calcium from bovine bone hydrolysates |
publisher |
Faculty of Science, Chiang Mai University |
publishDate |
2020 |
url |
https://epg.science.cmu.ac.th/ejournal/dl.php?journal_id=11193 http://cmuir.cmu.ac.th/jspui/handle/6653943832/69912 |
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