Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation

© 2020 Wiley Periodicals LLC. The effective enhancement of β-carotene production by Sporidiobolus pararoseus KM281507 was successfully achieved by random mutagenesis using ethyl methanesulfonate (EMS) combined with light irradiation. The mutant S. pararoseus E47 obtained from EMS mutagenesis produce...

Full description

Saved in:
Bibliographic Details
Main Authors: Atchara Manowattana, Charin Techapun, Thunnop Laokuldilok, Yuthana Phimolsiripol, Thanongsak Chaiyaso
Format: Journal
Published: 2020
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085942618&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69991
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-69991
record_format dspace
spelling th-cmuir.6653943832-699912020-10-14T08:28:42Z Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation Atchara Manowattana Charin Techapun Thunnop Laokuldilok Yuthana Phimolsiripol Thanongsak Chaiyaso Agricultural and Biological Sciences Chemical Engineering Chemistry © 2020 Wiley Periodicals LLC. The effective enhancement of β-carotene production by Sporidiobolus pararoseus KM281507 was successfully achieved by random mutagenesis using ethyl methanesulfonate (EMS) combined with light irradiation. The mutant S. pararoseus E47 obtained from EMS mutagenesis produced a promising yield of biomass, β-carotene, and total carotenoids under white light (1,000 Lux). The maximal biomass (19.76 g/L), β-carotene (128.97 mg/L), total carotenoids (179.72 mg/L), and β-carotene content (72%, w/w) of the total carotenoids were obtained. The β-carotene-rich carotenoids were extracted from yeast cells and encapsulated in alginate beads using a new encapsulation method to enhance its stability, revealing an effective concentration of 0.5% alginate + 1.0% vitamin C. The half-life of this β-carotene was 13 and 68 days for un-encapsulated and encapsulated + 1.0% vitamin C at 4°C with 1,000-Lux light irradiation, respectively. This result could uncover a potential alternative β-carotene source and its further application in foods, animal feeds, and cosmetic industries. Practical applications: The carotenoid extract of S. pararoseus mutant E47 was rich in β-carotene (72%, w/w). After extraction, the β-carotene-rich carotenoids were very sensitive to light and oxygen; thus, they were stabilized using a microencapsulation technique. To enhance their stability, vitamin C was added in the alginate phase making the β-carotene-rich carotenoid alginate beads (BCAB) highly stable and simply used for many applications including foods, feeds, cosmetics, and medicines. 2020-10-14T08:22:47Z 2020-10-14T08:22:47Z 2020-08-01 Journal 17454549 01458892 2-s2.0-85085942618 10.1111/jfpp.14596 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085942618&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/69991
institution Chiang Mai University
building Chiang Mai University Library
continent Asia
country Thailand
Thailand
content_provider Chiang Mai University Library
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Chemical Engineering
Chemistry
spellingShingle Agricultural and Biological Sciences
Chemical Engineering
Chemistry
Atchara Manowattana
Charin Techapun
Thunnop Laokuldilok
Yuthana Phimolsiripol
Thanongsak Chaiyaso
Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
description © 2020 Wiley Periodicals LLC. The effective enhancement of β-carotene production by Sporidiobolus pararoseus KM281507 was successfully achieved by random mutagenesis using ethyl methanesulfonate (EMS) combined with light irradiation. The mutant S. pararoseus E47 obtained from EMS mutagenesis produced a promising yield of biomass, β-carotene, and total carotenoids under white light (1,000 Lux). The maximal biomass (19.76 g/L), β-carotene (128.97 mg/L), total carotenoids (179.72 mg/L), and β-carotene content (72%, w/w) of the total carotenoids were obtained. The β-carotene-rich carotenoids were extracted from yeast cells and encapsulated in alginate beads using a new encapsulation method to enhance its stability, revealing an effective concentration of 0.5% alginate + 1.0% vitamin C. The half-life of this β-carotene was 13 and 68 days for un-encapsulated and encapsulated + 1.0% vitamin C at 4°C with 1,000-Lux light irradiation, respectively. This result could uncover a potential alternative β-carotene source and its further application in foods, animal feeds, and cosmetic industries. Practical applications: The carotenoid extract of S. pararoseus mutant E47 was rich in β-carotene (72%, w/w). After extraction, the β-carotene-rich carotenoids were very sensitive to light and oxygen; thus, they were stabilized using a microencapsulation technique. To enhance their stability, vitamin C was added in the alginate phase making the β-carotene-rich carotenoid alginate beads (BCAB) highly stable and simply used for many applications including foods, feeds, cosmetics, and medicines.
format Journal
author Atchara Manowattana
Charin Techapun
Thunnop Laokuldilok
Yuthana Phimolsiripol
Thanongsak Chaiyaso
author_facet Atchara Manowattana
Charin Techapun
Thunnop Laokuldilok
Yuthana Phimolsiripol
Thanongsak Chaiyaso
author_sort Atchara Manowattana
title Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
title_short Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
title_full Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
title_fullStr Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
title_full_unstemmed Enhancement of β-carotene-rich carotenoid production by a mutant Sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
title_sort enhancement of β-carotene-rich carotenoid production by a mutant sporidiobolus pararoseus and stabilization of its antioxidant activity by microencapsulation
publishDate 2020
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85085942618&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/69991
_version_ 1681752821115912192