EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2

Extracellular matrix metalloproteinase inducer (EMMPRIN) exhibits overexpression in various cancers and promotes cancer progression and metastasis via the interaction with its associated molecules. The scFv-M6-1B9 intrabody has a potential ability to reduce EMMPRIN cell surface expression. However,...

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Main Authors: Sangboonruang S., Thammasit P., Intasai N., Kasinrerk W., Tayapiwatana C., Tragoolpua K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84902024588&partnerID=40&md5=5393be25baf2d0a11e11aafe253e0f03
http://cmuir.cmu.ac.th/handle/6653943832/701
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-7012014-08-29T09:01:58Z EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2 Sangboonruang S. Thammasit P. Intasai N. Kasinrerk W. Tayapiwatana C. Tragoolpua K. Extracellular matrix metalloproteinase inducer (EMMPRIN) exhibits overexpression in various cancers and promotes cancer progression and metastasis via the interaction with its associated molecules. The scFv-M6-1B9 intrabody has a potential ability to reduce EMMPRIN cell surface expression. However, the subsequent effect of scFv-M6-1B9 intrabody-mediated EMMPRIN abatement on its related molecules, α3β1-integrin, MCT1, MMP-2 and MMP-9, is undefined. Our results demonstrated that the scFv-M6-1B9 intrabody efficiently decreased α3β1-integrin cell surface expression levels. In addition, intracellular accumulation of MCT1 and lactate were increased. These results lead to suppression of features characteristic for tumor progression, including cell migration, proliferation and invasion, in a colorectal cancer cell line (Caco-2) although there was no difference in MMP expression. Thus, EMMPRIN represents an attractive target molecule for the disruption of cancer proliferation and metastasis. An scFv-M6-1B9 intrabody-based approach could be relevant for cancer gene therapy.Cancer Gene Therapy advance online publication, 13 June 2014; doi:10.1038/cgt.2014.24. 2014-08-29T09:01:58Z 2014-08-29T09:01:58Z 2014 Article in Press 09291903 10.1038/cgt.2014.24 CGTHE http://www.scopus.com/inward/record.url?eid=2-s2.0-84902024588&partnerID=40&md5=5393be25baf2d0a11e11aafe253e0f03 http://cmuir.cmu.ac.th/handle/6653943832/701 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Extracellular matrix metalloproteinase inducer (EMMPRIN) exhibits overexpression in various cancers and promotes cancer progression and metastasis via the interaction with its associated molecules. The scFv-M6-1B9 intrabody has a potential ability to reduce EMMPRIN cell surface expression. However, the subsequent effect of scFv-M6-1B9 intrabody-mediated EMMPRIN abatement on its related molecules, α3β1-integrin, MCT1, MMP-2 and MMP-9, is undefined. Our results demonstrated that the scFv-M6-1B9 intrabody efficiently decreased α3β1-integrin cell surface expression levels. In addition, intracellular accumulation of MCT1 and lactate were increased. These results lead to suppression of features characteristic for tumor progression, including cell migration, proliferation and invasion, in a colorectal cancer cell line (Caco-2) although there was no difference in MMP expression. Thus, EMMPRIN represents an attractive target molecule for the disruption of cancer proliferation and metastasis. An scFv-M6-1B9 intrabody-based approach could be relevant for cancer gene therapy.Cancer Gene Therapy advance online publication, 13 June 2014; doi:10.1038/cgt.2014.24.
format Article
author Sangboonruang S.
Thammasit P.
Intasai N.
Kasinrerk W.
Tayapiwatana C.
Tragoolpua K.
spellingShingle Sangboonruang S.
Thammasit P.
Intasai N.
Kasinrerk W.
Tayapiwatana C.
Tragoolpua K.
EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
author_facet Sangboonruang S.
Thammasit P.
Intasai N.
Kasinrerk W.
Tayapiwatana C.
Tragoolpua K.
author_sort Sangboonruang S.
title EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
title_short EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
title_full EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
title_fullStr EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
title_full_unstemmed EMMPRIN reduction via scFv-M6-1B9 intrabody affects α3β1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2
title_sort emmprin reduction via scfv-m6-1b9 intrabody affects α3β1-integrin and mct1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line caco-2
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84902024588&partnerID=40&md5=5393be25baf2d0a11e11aafe253e0f03
http://cmuir.cmu.ac.th/handle/6653943832/701
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