Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice

Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Previously we showed that pretreatment of mice with CpG oligodeoxynucleotide (CpG ODN) 2 to 10 days prior to B. pseudomallei challenge conferred as high as 90% protection, but this window of protection w...

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Main Authors: Apichaya Puangpetch, Robert Anderson, Yan Y. Huang, Rasana W. Sermswan, Wanpen Chaicumpa, Stitaya Sirisinha, Surasakdi Wongratanacheewina
Other Authors: Khon Kaen University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/13746
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spelling th-mahidol.137462018-06-11T12:11:56Z Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice Apichaya Puangpetch Robert Anderson Yan Y. Huang Rasana W. Sermswan Wanpen Chaicumpa Stitaya Sirisinha Surasakdi Wongratanacheewina Khon Kaen University Dalhousie University Faculty of Medicine, Siriraj Hospital, Mahidol University Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Previously we showed that pretreatment of mice with CpG oligodeoxynucleotide (CpG ODN) 2 to 10 days prior to B. pseudomallei challenge conferred as high as 90% protection, but this window of protection was rather short. In the present study, we therefore aimed to prolong this protective window and to gain further insight into the mechanisms underlying the protection induced by CpG ODN against B. pseudomallei infection. It was found that the CpG ODN incorporated with cationic liposomes (DOTAP) but not zwitterionic liposomes (DOPC) provided complete protection against bacterial challenge. Although marked elevation of gamma interferon (IFN-γ) was found in the infected animals 2 days postinfection, it was significantly lowered by the DOTAP-plus-CpG ODN pretreatment. When appropriately activated, the phagocytic index and oxidative burst responses of neutrophils appeared not to be elevated. However, macrophages from stimulated mice showed higher levels of nitric oxide production and exhibited higher levels of antimicrobial activities, judging from lower numbers of viable intracellular bacteria. Taken together, our results demonstrate that DOTAP can enhance the protective window period of CpG ODN to at least 30 days and provide 100% protection against B. pseudomallei infection. The protective effect of DOTAP plus CpG ODN could provide an alternative approach to preventing this lethal infection, for which no vaccine is yet available. Copyright © 2012, American Society for Microbiology. 2018-06-11T04:37:21Z 2018-06-11T04:37:21Z 2012-05-01 Article Clinical and Vaccine Immunology. Vol.19, No.5 (2012), 675-683 10.1128/CVI.05545-11 1556679X 15566811 2-s2.0-84860334366 https://repository.li.mahidol.ac.th/handle/123456789/13746 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84860334366&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Apichaya Puangpetch
Robert Anderson
Yan Y. Huang
Rasana W. Sermswan
Wanpen Chaicumpa
Stitaya Sirisinha
Surasakdi Wongratanacheewina
Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
description Melioidosis is a severe disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Previously we showed that pretreatment of mice with CpG oligodeoxynucleotide (CpG ODN) 2 to 10 days prior to B. pseudomallei challenge conferred as high as 90% protection, but this window of protection was rather short. In the present study, we therefore aimed to prolong this protective window and to gain further insight into the mechanisms underlying the protection induced by CpG ODN against B. pseudomallei infection. It was found that the CpG ODN incorporated with cationic liposomes (DOTAP) but not zwitterionic liposomes (DOPC) provided complete protection against bacterial challenge. Although marked elevation of gamma interferon (IFN-γ) was found in the infected animals 2 days postinfection, it was significantly lowered by the DOTAP-plus-CpG ODN pretreatment. When appropriately activated, the phagocytic index and oxidative burst responses of neutrophils appeared not to be elevated. However, macrophages from stimulated mice showed higher levels of nitric oxide production and exhibited higher levels of antimicrobial activities, judging from lower numbers of viable intracellular bacteria. Taken together, our results demonstrate that DOTAP can enhance the protective window period of CpG ODN to at least 30 days and provide 100% protection against B. pseudomallei infection. The protective effect of DOTAP plus CpG ODN could provide an alternative approach to preventing this lethal infection, for which no vaccine is yet available. Copyright © 2012, American Society for Microbiology.
author2 Khon Kaen University
author_facet Khon Kaen University
Apichaya Puangpetch
Robert Anderson
Yan Y. Huang
Rasana W. Sermswan
Wanpen Chaicumpa
Stitaya Sirisinha
Surasakdi Wongratanacheewina
format Article
author Apichaya Puangpetch
Robert Anderson
Yan Y. Huang
Rasana W. Sermswan
Wanpen Chaicumpa
Stitaya Sirisinha
Surasakdi Wongratanacheewina
author_sort Apichaya Puangpetch
title Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
title_short Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
title_full Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
title_fullStr Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
title_full_unstemmed Cationic liposomes extend the immunostimulatory effect of CpG oligodeoxynucleotide against burkholderia pseudomallei infection in BALB/c mice
title_sort cationic liposomes extend the immunostimulatory effect of cpg oligodeoxynucleotide against burkholderia pseudomallei infection in balb/c mice
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/13746
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