A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility

• Main Authors: D. Japrung, S. Chusacultanachai, J. Yuvaniyama, P. Wilairat, Y. Yuthavong
• Other Authors: Thailand National Center for Genetic Engineering and Biotechnology
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/16291
• Institution: Mahidol University

Sufficient solubility of the active protein in aqueous solution is a prerequisite for crystallization and other structural studies of proteins. In this study, we have developed a simple and effective in vivo screening system to select for functionally active proteins with increased solubility by usi...