The chromosomal organization of the Plasmodium falciparum var gene family is conserved
The var gene family of Plasmodium falciparum encodes the protein PfEMP1 which is located on the surface of infected erythrocytes and is the receptor that mediates binding to ligands on endothelial cells. This family of proteins is responsible for antigenic variation and differences in binding phenot...
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th-mahidol.178912018-07-04T14:43:20Z The chromosomal organization of the Plasmodium falciparum var gene family is conserved Jennifer K. Thompson Justin P. Rubio Sonia Caruana Alan Brockman Mark E. Wickham Alan F. Cowman Walter Eliza Hall Inst. of Med. Res. Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology The var gene family of Plasmodium falciparum encodes the protein PfEMP1 which is located on the surface of infected erythrocytes and is the receptor that mediates binding to ligands on endothelial cells. This family of proteins is responsible for antigenic variation and differences in binding phenotype to ligands such as CD36 and ICAM1. We have compared the organization of the var gene family in three in vitro cloned lines of P. falciparum and show that most var genes are located in the subtelomeric region of each chromosome closely linked to the repetitive sequence rep20. While most chromosomes possess var genes in the subtelomeric region, in each in vitro cloned line there are some chromosomes that have deleted subtelomeric repetitive regions which include var genes. Comparison of the location of var genes in a field isolate showed that it does not have any detectable subtelomeric deletions as all chromosomes contain var genes and rep20 sequences. We have detected three chromosomes (4, 7 and 12) that contain var gene loci in more stable central regions and the position of these genes on chromosome 4 in the cloned lines analysed is conserved. The location of most of the var gene family in the subtelomeric region of the genome of P. falciparum has important implications for the generation of antigenic diversity of the PfEMP1 protein. 2018-07-04T07:40:21Z 2018-07-04T07:40:21Z 1997-07-01 Article Molecular and Biochemical Parasitology. Vol.87, No.1 (1997), 49-60 10.1016/S0166-6851(97)00041-8 01666851 2-s2.0-0030845471 https://repository.li.mahidol.ac.th/handle/123456789/17891 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0030845471&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Jennifer K. Thompson Justin P. Rubio Sonia Caruana Alan Brockman Mark E. Wickham Alan F. Cowman The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
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The var gene family of Plasmodium falciparum encodes the protein PfEMP1 which is located on the surface of infected erythrocytes and is the receptor that mediates binding to ligands on endothelial cells. This family of proteins is responsible for antigenic variation and differences in binding phenotype to ligands such as CD36 and ICAM1. We have compared the organization of the var gene family in three in vitro cloned lines of P. falciparum and show that most var genes are located in the subtelomeric region of each chromosome closely linked to the repetitive sequence rep20. While most chromosomes possess var genes in the subtelomeric region, in each in vitro cloned line there are some chromosomes that have deleted subtelomeric repetitive regions which include var genes. Comparison of the location of var genes in a field isolate showed that it does not have any detectable subtelomeric deletions as all chromosomes contain var genes and rep20 sequences. We have detected three chromosomes (4, 7 and 12) that contain var gene loci in more stable central regions and the position of these genes on chromosome 4 in the cloned lines analysed is conserved. The location of most of the var gene family in the subtelomeric region of the genome of P. falciparum has important implications for the generation of antigenic diversity of the PfEMP1 protein. |
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Walter Eliza Hall Inst. of Med. Res. |
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Walter Eliza Hall Inst. of Med. Res. Jennifer K. Thompson Justin P. Rubio Sonia Caruana Alan Brockman Mark E. Wickham Alan F. Cowman |
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Article |
author |
Jennifer K. Thompson Justin P. Rubio Sonia Caruana Alan Brockman Mark E. Wickham Alan F. Cowman |
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Jennifer K. Thompson |
title |
The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
title_short |
The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
title_full |
The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
title_fullStr |
The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
title_full_unstemmed |
The chromosomal organization of the Plasmodium falciparum var gene family is conserved |
title_sort |
chromosomal organization of the plasmodium falciparum var gene family is conserved |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/17891 |
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1763492984892948480 |