Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3

• Main Authors: Wanisa Salaemae, Muhammad Junaid, Chanan Angsuthanasombat, Gerd Katzenmeier
• Other Authors: Mahidol University. Institute of Molecular Biosciences. Laboratory of Molecular Virology
• Format: Article
• Language: English
• Published: 2015
• Subjects: Structure-guided; mutagenesis; active site residues; dengue virus; two-component; NS2B-NS3; protease; Open Access article
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/1833
• Institution: Mahidol University

Background The dengue virus two-component protease NS2B/NS3 mediates processing of the viral polyprotein precursor and is therefore an important determinant of virus replication. The enzyme is now intensively studied with a view to the structure-based development of antiviral inhibitors. Although 3...