Insights into the mechanism and regulation of pyruvate carboxylase by characterisation of a biotin-deficient mutant of the Bacillus thermodenitrificans enzyme

• المؤلفون الرئيسيون: Abdussalam Adina-Zada, Sarawut Jitrapakdee, Kathy H. Surinya, Matthew J. McIldowie, Matthew J. Piggott, W. Wallace Cleland, John C. Wallace, Paul V. Attwood
• مؤلفون آخرون: University of Western Australia
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/18988
• المؤسسة: Mahidol University

Pyruvate carboxylase is a biotin-dependent enzyme in which the biotin is carboxylated by a putative carboxyphosphate intermediate that is formed in a reaction between ATP and bicarbonate. The resultant carboxybiotin then transfers its carboxyl group to pyruvate to form oxaloacetate. In the Bacillus...